IGF3 敲低后鲤鱼（Cyprinus carpio）的长非编码 RNA 和 mRNA 表达谱。
Long noncoding RNA and mRNA expression profiles following igf3 knockdown in common carp, Cyprinus carpio.
机构信息
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, Jiangsu, China.
Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Wuxi 214081, Jiangsu, China.
出版信息
Sci Data. 2019 Feb 19;6:190024. doi: 10.1038/sdata.2019.24.
As a novel IGF system member, igf3 plays an important role in gonadal development of teleost fish. Although studies have reported the unusual expression of igf3 in fish gonad, whether the igf3 affects the expression of long noncoding RNAs (lncRNAs) in gonad remains unknown. In this study, an igf3 knockdown common carp (Cyprinus carpio) model was established by RNA interference. Then RNA sequencing of C. carpio gonad after igf3 knockdown was performed. A total of 327,169,410 and 306,305,018 clean reads were identified from control and igf3-dsRNA interference group, respectively. After a stringent filtering, RNA-seq yielded 14199 lncRNA and 106932 mRNA transcripts with 124 and 353 differentially expressed lncRNAs and mRNAs. Our dataset provides an extensive resource for understanding the potential regulatory molecular mechanism of igf3 in early stage of gonadal development in C. carpio.
作为 IGF 系统的新成员，igf3 在硬骨鱼的性腺发育中起着重要作用。尽管有研究报道 igf3 在鱼类性腺中的异常表达，但 igf3 是否影响性腺中长链非编码 RNA（lncRNA）的表达尚不清楚。在这项研究中，通过 RNA 干扰建立了 igf3 敲低的鲤鱼（Cyprinus carpio）模型。然后对 igf3 敲低后的 C. carpio 性腺进行 RNA 测序。分别从对照组和 igf3-dsRNA 干扰组中鉴定出 327169410 和 306305018 个清洁读数。经过严格筛选，RNA-seq 产生了 14199 个 lncRNA 和 106932 个 mRNA 转录本，其中有 124 个和 353 个差异表达的 lncRNA 和 mRNA。我们的数据集为理解 igf3 在 C. carpio 性腺早期发育中的潜在调控分子机制提供了广泛的资源。
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