State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Sun Yat-Sen University, Guangzhou, 510275, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong, 266237, China.
Sci Data. 2024 Oct 30;11(1):1181. doi: 10.1038/s41597-024-04016-2.
The giant freshwater prawn (Macrobrachium rosenbergii) has many advantages in aquaculture, such as fast growth rate, short breeding cycle and good nutrition, which makes it a freshwater shrimp with high economic value. Herein, high-quality chromosome-level genome of both female and male prawns were obtained by combining Illumina paired-end sequencing, PacBio single molecule sequencing technique and High-through chromosome conformation capture (Hi-C) technologies. In ZZ male prawn, a final contig assembly of 3118.58 Mb with a N50 length of 956,237 bp was obtained. In WW female prawn, a final contig assembly of 3333.31 Mb with a N50 length of 1,143,555 bp was obtained. The assembled genome sequences from prawns were anchored to 59 chromosomes. Moreover, the sex chromosomes including W chromosome and Z chromosome were generated in prawn with the length of 36.23 Mb and 27.33 Mb, respectively. The sequence similarity of Z chromosome and W chromosome reached to 74.90%. The high-quality genome resource will be useful for further molecular breeding and functional genomic research of giant freshwater prawns.
罗氏沼虾（Macrobrachium rosenbergii）在水产养殖中有许多优势，如生长速度快、繁殖周期短、营养价值高，是一种具有很高经济价值的淡水虾。本研究通过结合 Illumina 配对末端测序、PacBio 单分子测序技术和高通量染色体构象捕获（Hi-C）技术，获得了雌雄罗氏沼虾的高质量染色体水平基因组。在 ZZ 雄性虾中，最终组装得到的序列大小为 3118.58Mb，N50 长度为 956,237bp。在 WW 雌性虾中，最终组装得到的序列大小为 3333.31Mb，N50 长度为 1,143,555bp。虾的组装基因组序列被锚定到 59 条染色体上。此外，还生成了长度分别为 36.23Mb 和 27.33Mb 的性染色体，即 W 染色体和 Z 染色体。Z 染色体和 W 染色体的序列相似性达到 74.90%。该高质量基因组资源将有助于罗氏沼虾的进一步分子育种和功能基因组研究。
