School of Marine Sciences, State Key Laboratory for Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Zhuhai, 519000, China.
Zhejiang Institute of Freshwater Fisheries, Huzhou, 313000, China.
Sci Data. 2024 Aug 28;11(1):935. doi: 10.1038/s41597-024-03804-0.
The giant river prawn (Macrobrachium rosenbergii) is one of the most widely cultured crustacean species. In recent years, its aquaculture has faced challenges, including the degradation of germplasm resources and the emergence of viral diseases. Genomic information can be a valuable resource for developing molecular breeding programs for this important aquaculture species. Here we constructed a high-quality reference genome for M. rosenbergii by integrating Nanopore, Illumina, and high-throughput chromosome conformation capture (Hi-C) technologies. The final genome assembly is 3.18 Gb in size, with scaffold N50 and contig N50 of 62.73 Mb and 8.92 Mb, respectively. Approximately 98.6% of the assembled sequences were anchored into 59 pseudo-chromosomes. Benchmarking Universal Single-Copy Orthologs (BUSCO) benchmark of the genome assembly reached 94.5%. Repetitive sequences comprise 43.77% of the assembled genome, and 17,436 protein-coding genes were annotated. The high-quality genome of M. rosenbergii will empower molecular breeding efforts and provide invaluable resources for comparative genomic analysis of decapod species.
罗氏沼虾是最广泛养殖的甲壳类动物之一。近年来，其水产养殖面临着种质资源退化和病毒性疾病出现等挑战。基因组信息可以成为为这一重要水产养殖物种开发分子育种计划的宝贵资源。在这里，我们通过整合纳米孔、Illumina 和高通量染色体构象捕获 (Hi-C) 技术，为罗氏沼虾构建了一个高质量的参考基因组。最终基因组组装大小为 3.18Gb，支架 N50 和 contig N50 分别为 62.73Mb 和 8.92Mb。大约 98.6%的组装序列被锚定到 59 条假染色体上。基因组组装的通用单拷贝同源物 (BUSCO) 基准测试达到了 94.5%。重复序列占组装基因组的 43.77%，注释了 17436 个蛋白质编码基因。罗氏沼虾的高质量基因组将为分子育种工作提供支持，并为十足目物种的比较基因组分析提供宝贵资源。
