Moore J V, Keene J P, Land E J
Br J Radiol. 1986 Mar;59(699):257-61. doi: 10.1259/0007-1285-59-699-257.
Oedema and necrosis of murine tail skin have been measured after intravenous injection of haematoporphyrin derivative ("Photofrin", or PhI) followed 24 h later by graded exposures of the tail to full-spectrum visible light from a quartz-halogen lamp. End-points were degree of oedema and the proportion of mice in a dose-group that developed skin necrosis and tail atrophy. Oedema developed within 24 h of illumination and was a function of PhI dose and duration of light exposure. Onset of necrosis occurred after a minimum of 5 days and onset time was an inverse function of exposure time. Probit plots of proportion of necroses versus light exposure yielded values for ND50 (exposure corresponding to 50% incidence of necrosis) and l/slope. At the high but non-toxic dose of 2 mg PhI/mouse, ND50 was 18 min, l/slope 4 min, for pigmented BDF1 mice. Halving the PhI dose increased ND50 by a factor of 1.9. Albino BALB/c mice were markedly more sensitive to 2 mg PhI plus light than BDF1 mice: the ND50 was 7 min. Temporary occlusion of the blood supply to the tail (10 min before and during illumination) abrogated totally the oedematous and necrotic reactions to photodynamic therapy.
在静脉注射血卟啉衍生物（“光卟啉”，或PhI）后24小时，将小鼠尾巴暴露于石英卤钨灯的全光谱可见光下进行分级照射，之后测量小鼠尾巴皮肤的水肿和坏死情况。终点指标为水肿程度以及剂量组中发生皮肤坏死和尾巴萎缩的小鼠比例。水肿在光照后24小时内出现，是PhI剂量和光照持续时间的函数。坏死至少在5天后发生，发病时间与暴露时间呈反比。坏死比例与光照时间的概率图得出了ND50（对应50%坏死发生率的暴露时间）和1/斜率的值。对于有色BDF1小鼠，在2 mg PhI/小鼠的高但无毒剂量下，ND50为18分钟，1/斜率为4分钟。将PhI剂量减半会使ND50增加1.9倍。白化BALB/c小鼠对2 mg PhI加光照的敏感性明显高于BDF1小鼠：ND50为7分钟。在光照前和光照期间暂时阻断尾巴的血液供应（10分钟）完全消除了对光动力疗法的水肿和坏死反应。
