Zhou C N, Yang W Z, Ding Z X, Wang Y X, Shen H, Fan X J, Ha X W
Adv Exp Med Biol. 1985;193:105-9. doi: 10.1007/978-1-4613-2165-1_12.
A light microscopic investigation of the biological effects of photodynamic therapy using hematoporphyrin derivative and red light irradiation has been carried out. HpD alone, even used at a dose of 50 mg/kg, did not cause histologically discernible changes. The irradiation alone used in this experiment caused only slight or moderate dilatation and congestion of blood vessels, which disappeared completely within several hours after light exposure. In the PDT treated mouse ears severe degeneration and necrosis of skin tissue occurred within 1-2 days. The importance of adequate drug and light doses and proper interval chosen for obtaining best therapeutic effects and protection of surrounding normal tissues was pointed out. The possible mechanism of the PDT action in vivo was briefly discussed.
利用血卟啉衍生物和红光照射进行光动力疗法的生物学效应的光学显微镜研究已经展开。单独使用血卟啉衍生物，即使剂量为50mg/kg，也未引起组织学上可辨别的变化。本实验中单独的照射仅引起血管轻微或中度扩张和充血，在光照后数小时内完全消失。在接受光动力疗法治疗的小鼠耳朵中，皮肤组织在1-2天内发生严重变性和坏死。指出了选择合适的药物和光照剂量以及适当的间隔时间对于获得最佳治疗效果和保护周围正常组织的重要性。简要讨论了光动力疗法在体内作用的可能机制。
