Botany Department, Faculty of Science, Tanta University, Tanta, Gharbia Governorate, Egypt.
F1000Res. 2024 Jan 18;12:262. doi: 10.12688/f1000research.130979.1. eCollection 2023.
Salt stress, a significant environmental problem was studied in barley cultivars Giza 124 and Giza 119 at various stages (seedling, pre-flowering, and yield). This study aimed to investigate the impact of salt stress on these cultivars, examine the effects of polyamine precursors (arginine, methionine, and ornithine) on their response to salt stress, and assess the efficacy of antioxidants (glutathione and ascorbic acid) in alleviating the harmful effects of salt stress on barley plants.
Barley grains were germinated and subjected to salinity stress, with subsequent treatment using glutathione, ascorbic acid, or an amino acid mixture. Growth criteria, photosynthetic pigments, metabolites, antioxidant enzymes, mineral content, and polyamines were analyzed.
The impact of 100Mm NaCl, with or without glutathione, ascorbic acid, or amino acid mixtures, on various physiological parameters in G124 and G119 were investigated. The levels of chlorophyll a, chlorophyll b, and carotenoids significantly varied under different treatments. For instance, chlorophyll a in G 124 exhibited a 23% reduction under salt stress compared to the control, while the addition of glutathione mitigated this effect, resulting in a 17% increase compared to the NaCl treatment. Similar trends were observed for chlorophyll b and carotenoids. At the yield stage, both cultivars demonstrated a significant decrease in the the weight of grains per plant under salinity, which was alleviated by the addition of ascorbic acid, glutathione, or amino acid mixtures.
The application of glutathione, ascorbic acid, or an amino acid mixture mitigated the adverse effects of salt stress on various parameters. The results highlight the potentail of these compounds in enhancing plant tolerance to salinity stress and offer insights into the physiological response of barley cultivars under adverse conditions.
在大麦品种 Giza 124 和 Giza 119 的幼苗期、花期前和产量期等不同阶段，研究了盐胁迫这一重要的环境问题。本研究旨在探讨盐胁迫对这些品种的影响，研究多胺前体（精氨酸、蛋氨酸和鸟氨酸）对其盐胁迫响应的影响，并评估抗氧化剂（谷胱甘肽和抗坏血酸）在缓解盐胁迫对大麦植株的有害影响方面的效果。
大麦种子发芽后进行盐胁迫处理，随后用谷胱甘肽、抗坏血酸或氨基酸混合物进行处理。分析生长指标、光合色素、代谢物、抗氧化酶、矿物质含量和多胺。
研究了 100mM NaCl 及其与谷胱甘肽、抗坏血酸或氨基酸混合物联合处理对 G124 和 G119 不同生理参数的影响。不同处理下叶绿素 a、叶绿素 b 和类胡萝卜素的水平差异显著。例如，盐胁迫下 G124 的叶绿素 a 含量比对照降低了 23%，而添加谷胱甘肽则减轻了这种影响，与 NaCl 处理相比增加了 17%。叶绿素 b 和类胡萝卜素也呈现出类似的趋势。在产量期，两个品种的单株粒重均因盐胁迫而显著降低，添加抗坏血酸、谷胱甘肽或氨基酸混合物可缓解这种降低。
谷胱甘肽、抗坏血酸或氨基酸混合物的应用减轻了盐胁迫对各种参数的不利影响。研究结果强调了这些化合物在增强植物对盐胁迫的耐受性方面的潜力，并为了解大麦品种在不利条件下的生理响应提供了新的思路。
