Department of Botany, University of Delhi, Delhi, India.
Department of Botany, Banaras Hindu University, Varanasi, India.
Int J Phytoremediation. 2023;25(4):403-414. doi: 10.1080/15226514.2022.2086215. Epub 2022 Jun 25.
L. is an important medicinal and food plant enormously affected by salinity in terms of its growth and quality. This experiment investigates ameliorative potential of NO donor sodium nitroprusside (SNP) on chromosomal aberrations and physiological parameters in L. roots exposed to salinity stress. Roots with different concentrations of NaCl (25, 50, and 100 mM) alone, and in combination with 100 µM SNP were analyzed for mitotic aberrations, DNA damage, proline, malondialdehyde (MDA) content, and ascorbate-glutathione (AsA-GSH) cycle after 120 h of salinity treatments. Results revealed that salinity stress increased chromosomal aberrations, MDA, proline accumulation, and severely hampered the AsA-GSH cycle function. The comet assay revealed a significant ( 0.05) enhancement in tail length (4.35 ± 0.05 µm) and olive tail moment (3.19 ± 0.04 µm) at 100 mM NaCl exposure. However, SNP supplementation decreased total percent abnormalities, while increased the prophase, metaphase, anaphase, and telophase indexes. Moreover, ascorbate peroxidase and glutathione reductase activities increased with AsA/DHA and GSH/GSSG ratios, respectively. Results suggest that SNP supplementation alleviates salinity stress responses by improving AsA-GSH cycle and proline accumulation. Based on present findings, NO supplementation could be recommended as a promising approach for sustainable crop production under salinity stress.
L. 是一种重要的药用和食用植物，其生长和品质会受到盐分的极大影响。本实验研究了一氧化氮供体硝普钠（SNP）对盐胁迫下 L. 根染色体畸变和生理参数的改良潜力。用不同浓度的 NaCl（25、50 和 100 mM）单独以及与 100 μM SNP 组合处理 120 h 后，分析了根尖的有丝分裂畸变、DNA 损伤、脯氨酸、丙二醛（MDA）含量和抗坏血酸-谷胱甘肽（AsA-GSH）循环。结果表明，盐胁迫增加了染色体畸变、MDA、脯氨酸积累，并严重阻碍了 AsA-GSH 循环功能。彗星试验显示，在 100 mM NaCl 暴露下，尾长（4.35±0.05 μm）和橄榄尾矩（3.19±0.04 μm）显著增加（P<0.05）。然而，SNP 的补充降低了总异常百分率，同时增加了前期、中期、后期和末期指数。此外，抗坏血酸过氧化物酶和谷胱甘肽还原酶的活性分别随着 AsA/DHA 和 GSH/GSSG 比值的增加而增加。结果表明，SNP 的补充通过改善 AsA-GSH 循环和脯氨酸积累来缓解盐胁迫反应。基于目前的发现，NO 的补充可以作为一种有前途的方法，用于在盐胁迫下可持续的作物生产。
