Chinbat Odgerel, Erdenetsog Purevdulam, Tuvshintur Buyankhuu, Gantumur Anuujin, Burenjargal Munkhjargal, Chimeddorj Battogtokh, Janlav Munkhtsetseg
Department of Biochemistry, School of BioMedicine Mongolian National University of Medical Sciences Ulaanbaatar Mongolia.
Department of Microbiology and Infection Prevention Control, School of BioМedicine Mongolian National University of Medical Sciences Ulaanbaatar Mongolia.
Food Sci Nutr. 2024 Aug 13;12(10):7877-7884. doi: 10.1002/fsn3.4391. eCollection 2024 Oct.
Xylooligosaccharides (XOS) are prebiotics of significant biological value that can be obtained through cost-effective purification of agricultural waste. The present research featured in vitro and in vivo investigation of prebiotic effects of xylooligosaccharides derived from wheat bran powder and brewer's spent grain. Prebiotic activity of , and spp. was investigated in vitro using standard selective media. 16S rRNA quantitative PCR used for in vitro and in vivo investigation quantified relative abundance of spp., spp., and in samples of fecal matter, cecal content, and intestinal tissue. Research revealed a favorable association between XOS concentration and both bacterial count and diameter of resultant colonies. The standard strain of showed no noticeable effect on growth rate. spp. proliferation in intestinal tissue was validated via in vivo tests using XOS obtained from wheat bran powder and brewer's spent grain. Findings indicated increased prevalence of the species and the presence of XOS showed a protective function in preserving the structural integrity of intestinal mucus secretions. The presence of XOS in food indicated direct association with proliferation of spp. and spp. Study results suggest that XOS extracted through enzymatic hydrolysis in Mongolian food industry by-products such as wheat bran products and brewer's spent grain exhibit prebiotic properties that justify XOS manufacture on a large scale and incorporation of XOS as nutritional enhancement in food products and pharmaceuticals.
低聚木糖（XOS）是具有重要生物学价值的益生元，可通过对农业废弃物进行经济高效的纯化获得。本研究对从麦麸粉和啤酒糟中提取的低聚木糖的益生元效应进行了体外和体内研究。使用标准选择性培养基在体外研究了双歧杆菌属、嗜酸乳杆菌属和双歧杆菌属的益生元活性。用于体外和体内研究的16S rRNA定量PCR对粪便、盲肠内容物和肠道组织样本中双歧杆菌属、嗜酸乳杆菌属和双歧杆菌属的相对丰度进行了量化。研究发现低聚木糖浓度与细菌数量及所得菌落直径之间存在良好的关联。双歧杆菌的标准菌株对生长速率没有明显影响。通过使用从麦麸粉和啤酒糟中获得的低聚木糖进行体内试验，验证了肠道组织中嗜酸乳杆菌属的增殖情况。研究结果表明嗜酸乳杆菌属物种的患病率增加，并且低聚木糖的存在对维持肠道黏液分泌物的结构完整性具有保护作用。食物中低聚木糖的存在表明与双歧杆菌属和嗜酸乳杆菌属的增殖直接相关。研究结果表明，在蒙古食品工业副产品如麦麸产品和啤酒糟中通过酶水解提取的低聚木糖具有益生元特性，这证明了大规模生产低聚木糖并将其作为食品和药品中的营养强化剂的合理性。
