Yu Xiuhua, Yin Jianyuan, Li Lin, Luan Chang, Zhang Jian, Zhao Chunfang, Li Shengyu
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P.R. China.
The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130021, P.R. China.
J Microbiol Biotechnol. 2015 Jul;25(7):1084-92. doi: 10.4014/jmb.1501.01022.
In the present work, the in vitro prebiotic activity of xylooligosaccharides (XOS) derived from corn cobs combined with Lactobacillus plantarum, a probiotic microorganism, was determined. These probiotics exhibited different growth characteristics depending on strain specificity. L. plantarum S2 cells were denser and their growth rates were higher when cultured on XOS. Acetate was found to be the major short-chain fatty acid produced as the end-product of fermentation, and its amount varied from 1.50 to 1.78 mg/ml. The antimicrobial activity of XOS combined with L. plantarum S2 was determined against gastrointestinal pathogens. The results showed that XOS proved to be an effective substrate, enhancing antimicrobial activity for L. plantarum S2. In vivo evaluation of the influence of XOS and L. plantarum S2, used both alone and together, on the intestinal microbiota in a mouse model showed that XOS combined with L. plantarum S2 could increase the viable lactobacilli and bifidobacteria in mice feces and decrease the viable Enterococcus, Enterobacter, and Clostridia spp. Furthermore, in the in vitro antioxidant assay, XOS combined with L. plantarum S2 possessed significant 2,2-diphenyl-1- picrylhydrazyl, 2,2'-azino-bis, and superoxide anion radical-scavenging activities, and the combinations showed better antioxidant activity than either XOS or L. plantarum S2 alone.
在本研究中，测定了从玉米芯中提取的低聚木糖（XOS）与益生菌植物乳杆菌组合后的体外益生元活性。这些益生菌根据菌株特异性表现出不同的生长特性。当在XOS上培养时，植物乳杆菌S2细胞更密集，其生长速率更高。发现乙酸是作为发酵终产物产生的主要短链脂肪酸，其含量在1.50至1.78mg/ml之间变化。测定了XOS与植物乳杆菌S2组合对胃肠道病原体的抗菌活性。结果表明，XOS被证明是一种有效的底物，可增强植物乳杆菌S2的抗菌活性。在小鼠模型中对单独使用和联合使用XOS和植物乳杆菌S2对肠道微生物群影响的体内评估表明，XOS与植物乳杆菌S2组合可增加小鼠粪便中活的乳酸杆菌和双歧杆菌数量，并减少活的肠球菌、肠杆菌和梭菌属。此外，在体外抗氧化试验中，XOS与植物乳杆菌S2组合具有显著的2,2-二苯基-1-苦基肼、2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）和超氧阴离子自由基清除活性，并且组合显示出比单独的XOS或植物乳杆菌S2更好的抗氧化活性。
