Chmykhalo V K, Shidlovskii Y V, Lebedeva L A, Schedl P, Giordano E
Institute of Gene Biology, Russian Academy of Sciences, Laboratory of Gene Expression Regulation in Development, Moscow, Russia.
Princeton University, Princeton, USA.
Dokl Biochem Biophys. 2024 Dec;519(1):588-592. doi: 10.1134/S160767292460088X. Epub 2024 Oct 31.
The phenotypic manifestations of increased expression of the Bap170 and e(y)3 (SAYP) genes in D. melanogaster were analyzed. Using the wing disc model, we show that moderate co-expression of Bap170 and e(y)3 genes in wing discs leads to abnormalities in wing veining. which was probably caused by suppression of EGFR/Ras/MAPK signaling pathways. Strong induction of co-expression of the above genes in wing discs leads to complete suppression of wing development in adults. Ubiquitous co-expression of Bap170 and e(y)3 is lethal at the 1st instar larval stage and leads to the formation of melanotic tumors. The above phenotypes are observed exclusively when Bap170 and e(y)3 are co-expressed. This evidence suggests a robust synergistic effect of the combined action of these genes, which is manifested in the hyperactivity of cell proliferation and differentiation.
分析了黑腹果蝇中Bap170和e(y)3（SAYP）基因表达增加的表型表现。利用翅盘模型，我们发现翅盘中Bap170和e(y)3基因的适度共表达会导致翅脉异常，这可能是由EGFR/Ras/MAPK信号通路的抑制引起的。翅盘中上述基因共表达的强烈诱导导致成虫翅发育完全受抑制。Bap170和e(y)3的普遍共表达在一龄幼虫阶段是致死的，并导致黑色素瘤的形成。仅当Bap170和e(y)3共表达时才会观察到上述表型。这一证据表明这些基因联合作用具有强大的协同效应，表现为细胞增殖和分化的过度活跃。
