Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.
Cancer Research Institute Ghent, Ghent, Belgium.
Nat Commun. 2024 Oct 31;15(1):9410. doi: 10.1038/s41467-024-53279-1.
Bacterial extracellular vesicles (BEVs) enable communication between bacteria and their natural habitats, including multicellular organisms such as humans. Consequently, the study of BEVs has rapidly gained attention with recent research raising the prospect of developing BEVs as biomarkers and treatments to manage (mal)functioning of natural habitats. Although diverse technologies are available, the composition of their source, their heterogeneity in biophysical and biochemical features, and their multifaceted cargo composition challenges the analysis of BEVs. To map current practices in BEV research, we analyzed 845 publications released in 2015-2021, reporting 3338 BEV-related experiments. The extracted data are accessible via the publicly available EV-TRACK knowledgebase ( https://evtrack.org/ ). We identify the need for transparent reporting, delineate knowledge gaps, outline available best practices and define areas in need of guidance to ensure advances in BEV research and accelerate BEV applications.
细菌细胞外囊泡（BEVs）使细菌与其自然栖息地（包括人类等多细胞生物）之间能够进行交流。因此，随着最近的研究提出将 BEVs 开发为生物标志物和治疗方法来管理（异常）自然栖息地功能的前景，BEVs 的研究迅速受到关注。尽管有多种技术可用，但由于其来源组成复杂、生物物理和生化特征存在异质性以及其多方面的货物组成，BEVs 的分析具有挑战性。为了绘制 BEV 研究的当前实践图谱，我们分析了 2015 年至 2021 年发布的 845 篇出版物，报告了 3338 项与 BEV 相关的实验。通过可公开访问的 EV-TRACK 知识库（https://evtrack.org/）可以访问提取的数据。我们确定了需要透明报告、划定知识空白、概述可用的最佳实践并确定需要指导的领域，以确保 BEV 研究的进展并加速 BEV 的应用。
