Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-902, Brazil.
Sci Rep. 2024 Nov 1;14(1):26250. doi: 10.1038/s41598-024-77919-0.
Accurate bacterial identification is essential for determining the causative agent of an infection, thus facilitating appropriate treatment and management strategies in both human and animal health contexts. Some species in the Vibrio genus are recognized pathogens, associated with a variety of infections. However, identification of these bacteria is oftentimes controversial. Therefore, we aimed to evaluate different identification approaches in terms of their reliability in distinguishing Vibrio species. To achieve this, we selected a set of 40 Vibrio isolates previously recovered from water and floating plastic samples in a large bay environment and identified them employing MALDI-TOF mass spectrometry, and rrs and pyrH gene sequencing. A subset of isolates was also submitted to whole genome sequencing. Overall, MALDI-TOF was found to be a fast-screening methodology for identification, notably at genus-level. However, for better species discrimination, pyrH gene sequencing stood out as a more reliable tool in contrast to rrs gene sequencing and MALDI-TOF, as corroborated by whole genome sequencing analysis.
准确的细菌鉴定对于确定感染的病原体至关重要，从而有助于在人类和动物健康环境中制定适当的治疗和管理策略。弧菌属中的一些物种是公认的病原体，与多种感染有关。然而，这些细菌的鉴定时常存在争议。因此，我们旨在评估不同的鉴定方法在区分弧菌物种方面的可靠性。为了实现这一目标，我们选择了一组 40 株先前从一个大型海湾环境中的水样和漂浮塑料样本中分离出的弧菌分离株，采用 MALDI-TOF 质谱法和 rrs 和 pyrH 基因测序法对其进行鉴定。部分分离株也进行了全基因组测序。总的来说，MALDI-TOF 被发现是一种快速的鉴定筛选方法，尤其是在属水平上。然而，为了更好地区分物种，pyrH 基因测序比 rrs 基因测序和 MALDI-TOF 更可靠，这一点得到了全基因组测序分析的证实。
