UPRES EA-4438, Physiopathologie et Médecine Translationnelle, Laboratoire de Bactériologie de la Faculté de Médecine, Université de Strasbourg, Hôpitaux Universitaires de Strasbourg, 3 Rue Koeberlé, 67000 Strasbourg, France.
Microbiol Res. 2012 Apr 20;167(4):226-30. doi: 10.1016/j.micres.2011.09.002. Epub 2011 Oct 19.
The aim of this study was to discriminate 30 Vibrio strains isolated from two wastewater treatment plants from Agadir, Morocco by two molecular typing methods, pulsed-field gel electrophoresis (PFGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Out of the 30 strains of Vibrio examined in this study, 5 isolates could not be typed by PFGE and consistently appeared as a smear on the gel. In general, high genetic biodiversity among the Vibrio strains was found regardless to the isolation source. The results of MALDI TOF analysis show a high congruence of strain grouping demonstrating the accuracy and reliability of MALDI-TOF MS.
本研究的目的是通过两种分子分型方法，即脉冲场凝胶电泳（PFGE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS），对从摩洛哥阿加迪尔的两个废水处理厂分离的 30 株弧菌进行区分。在本研究中检查的 30 株弧菌中，有 5 株不能通过 PFGE 进行分型，并且在凝胶上始终显示为一条污迹。通常，无论分离源如何，都发现弧菌菌株之间具有很高的遗传多样性。MALDI TOF 分析的结果显示，菌株分组高度一致，证明了 MALDI-TOF MS 的准确性和可靠性。
