Canellas Anna Luiza Bauer, de Oliveira Bruno Francesco Rodrigues, Laport Marinella Silva
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-902, Brazil.
Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, 24210-130, Brazil.
Braz J Microbiol. 2025 May 22. doi: 10.1007/s42770-025-01700-2.
Vibrio spp. are endowed with high metabolic diversity, being known for their pathogenicity and ability to acquire and disseminate resistance genes. Here, non-cholera Vibrio spp. were isolated from water samples in Guanabara Bay (GB, Brazil), a recreational estuary. Thirty-five strains non-susceptible to antimicrobials were investigated regarding their pathogenic and resistance profiles. Genes encoding for hemolysins and secretion systems were among the detected virulence traits. Beta-lactam, aminoglycoside, and quinolone resistance genes were investigated in strains phenotypically resistant to these classes, revealing the carriage of the bla and aac(6')-Ie-aph(2')-Ia genes in 50% and 11.1% of the tested strains, respectively. Integrons (classes 1 and 3) and heavy metal resistance genes were detected, likely reflecting local pollution levels. Genome sequencing of five strains revealed further insights regarding their associated resistome and virulome, confirming phenotypic-derived results. Our findings imply that pathogenic and antimicrobial-resistant Vibrio spp. are found in the GB, thus posing a risk to public health.
弧菌属具有高度的代谢多样性，以其致病性以及获取和传播耐药基因的能力而闻名。在此，从巴西瓜纳巴拉湾（GB）一个用于休闲娱乐的河口的水样中分离出非霍乱弧菌属。对35株对抗菌药物不敏感的菌株进行了致病性和耐药性分析。检测到的毒力特征包括编码溶血素和分泌系统的基因。对表型上对β-内酰胺类、氨基糖苷类和喹诺酮类耐药的菌株研究了其耐药基因，结果显示分别有50%和11.1%的受试菌株携带bla和aac(6')-Ie-aph(2')-Ia基因。检测到了整合子（1类和3类）和重金属耐药基因，这可能反映了当地的污染水平。对五株菌株进行全基因组测序，进一步揭示了其相关的耐药组和毒力组，证实了基于表型得出的结果。我们的研究结果表明，在瓜纳巴拉湾发现了具有致病性和抗药性的弧菌属，从而对公众健康构成风险。
