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犬肥大细胞瘤中 Beclin-1 和 LC3B 的表达：自噬的免疫超微结构和免疫组织化学研究。

Beclin-1 and LC3B expression in canine mast cell tumours: an immuno-ultrastructural and immunohistochemical study of autophagy.

机构信息

Laboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.

Department of Veterinary Medicine, Università degli Studi di Teramo (UNITE), Teramo, Italy.

出版信息

Vet Q. 2024 Dec;44(1):1-15. doi: 10.1080/01652176.2024.2419585. Epub 2024 Nov 1.

DOI:10.1080/01652176.2024.2419585
PMID:39483060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11536674/
Abstract

Mast cell tumours (MCTs) are common malignant neoplasms in dogs, for which prognosis and therapeutic decisions are based on histological features and proliferation markers. Autophagy is a cellular catabolic process responsible for degrading cytoplasmic components to maintain homeostasis, alterations in which are frequently linked to tumour growth and progression. This study was conducted to investigate the occurrence of autophagy in canine MCTs and to verify its value as a prognostic indicator for dogs with the disease. Beclin-1 and LC3B expressions were investigated using immunohistochemistry, and autophagy was ultrastructurally characterised. The autophagic phenomenon was successfully visualised in neoplastic mast cells under transmission electron and immunoelectron microscopy. MCTs from dogs that died due to the disease showed higher positivity for Beclin-1 and dogs with MCTs presenting a LC3B granular immunohistochemical pattern had a significantly shorter post-surgical survival. The occurrence of autophagy is an indicator of poor prognosis. Future studies are needed to elucidate the specific mechanisms and open new opportunities to treatments targeting this cancer cell advantage.

摘要

肥大细胞瘤（MCT）是犬类常见的恶性肿瘤，其预后和治疗决策基于组织学特征和增殖标志物。自噬是一种细胞分解代谢过程，负责降解细胞质成分以维持体内平衡，其改变通常与肿瘤生长和进展有关。本研究旨在调查犬类 MCT 中自噬的发生情况，并验证其作为该疾病犬预后指标的价值。使用免疫组织化学法研究了 Beclin-1 和 LC3B 的表达，并对自噬进行了超微结构特征分析。在透射电子和免疫电子显微镜下成功观察到了肿瘤肥大细胞中的自噬现象。因该疾病而死亡的犬的 MCT 中 Beclin-1 的阳性率更高，而表现出 LC3B 颗粒状免疫组织化学模式的 MCT 犬手术后的存活时间明显更短。自噬的发生是预后不良的指标。未来的研究需要阐明具体的机制，并为针对这种癌细胞优势的治疗方法开辟新的机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/c370ac3cc8af/TVEQ_A_2419585_F0008_C.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/d6200635cc01/TVEQ_A_2419585_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/a228c9977aad/TVEQ_A_2419585_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/5d802a711f2b/TVEQ_A_2419585_F0007_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/c370ac3cc8af/TVEQ_A_2419585_F0008_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/0d7bab2befb7/TVEQ_A_2419585_F0001_C.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/2148fc24037b/TVEQ_A_2419585_F0002_C.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/cc962ce7c361/TVEQ_A_2419585_F0004_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/d6200635cc01/TVEQ_A_2419585_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/a228c9977aad/TVEQ_A_2419585_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/5d802a711f2b/TVEQ_A_2419585_F0007_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2766/11536674/c370ac3cc8af/TVEQ_A_2419585_F0008_C.jpg

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