Ji C, Li M H, Li J L, Lu S J
Carcinogenesis. 1986 Feb;7(2):301-3. doi: 10.1093/carcin/7.2.301.
Nitrosomethylisoamylamine (NMIA), a carcinogenic N-nitroso compound was synthesized from isoamylamine (IAA) in a glucose-ammonium nitrate medium after several days' incubation with fungi and subsequent nitrosation with sodium nitrate. The nitrosamine was not produced by control reactions which lacked either IAA or fungi. The yield of NMIA varied with the length of incubation after inoculation with the fungi, and with the concentrations of IAA and NaNO2, the duration of nitrosation, the pH of the medium and the species of the fungi. The optimum conditions for the formation of the nitrosamine are reported.
亚硝基甲基异戊胺（NMIA）是一种致癌的N-亚硝基化合物，在葡萄糖-硝酸铵培养基中，异戊胺（IAA）与真菌孵育数天后，再用硝酸钠进行亚硝化反应，从而合成得到该物质。在缺少IAA或真菌的对照反应中不会产生亚硝胺。NMIA的产量随接种真菌后的孵育时间、IAA和亚硝酸钠的浓度、亚硝化反应的持续时间、培养基的pH值以及真菌的种类而变化。本文报道了亚硝胺形成的最佳条件。
