Ji C A, Li M X, Li J L, Zhao S J, Wang G H, He Y Z
Sci Sin B. 1985 Nov;28(11):1188-96.
Methylisoamylnitrosamine, a carcinogenic N-nitroso compound, has been formed in glucose ammonium nitrate medium containing 150 mg of isoamylamine (a primary amine) inoculated with a common fungus (Fusarium moniliforme Sheldon), to which 400 mg NaNO2 are added after incubation for 7-8 days at 28 degrees C. No such compound has been found in control samples without fungi or isoamylamine. This paper reports a method of experimental synthesis of the nitrosamine by F. moniliforme strains 82-01 and 83-01, using isoamylamine and NaNO2, as well as the analysis of the compound by GC/MS and GC/TEA.
甲基异戊基亚硝胺是一种致癌的N-亚硝基化合物，在含有150毫克异戊胺（一种伯胺）的葡萄糖硝酸铵培养基中，接种常见真菌（串珠镰刀菌谢尔顿）后形成。在28℃下培养7-8天后添加400毫克亚硝酸钠。在没有真菌或异戊胺的对照样品中未发现此类化合物。本文报道了由串珠镰刀菌82-01和83-01菌株利用异戊胺和亚硝酸钠实验合成亚硝胺的方法，以及用气相色谱/质谱联用仪和气相色谱/热电子捕获检测器对该化合物进行分析。
