Santella R M, Lin C D, Dharmaraja N
Carcinogenesis. 1986 Mar;7(3):441-4. doi: 10.1093/carcin/7.3.441.
Monoclonal antibodies have been developed which specifically recognize protein modified by 7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE-I). These antibodies have been characterized as to sensitivity and specificity by an enzyme-linked immunosorbent assay. They react with BPDE-I-modified proteins and do not cross-react with non-modified protein. All the antibodies have higher sensitivity toward BPDE-I-tetraols than to BPDE-I-modified protein. They cross-react with BPDE-I-deoxyguanosine, BPDE-I-DNA and BPDE-II-rabbit serum albumin. Because of the high antibody sensitivity for tetraols, an immunoassay was developed for quantitating DNA and protein adducts based on release of tetraols from modified samples. This approach was validated using radiolabeled BPDE-I modified DNA and protein.
已经开发出了单克隆抗体，它们能够特异性识别由7β,8α-二羟基-9α,10α-环氧-7,8,9,10-四氢苯并[a]芘（BPDE-I）修饰的蛋白质。通过酶联免疫吸附测定法对这些抗体的敏感性和特异性进行了表征。它们与BPDE-I修饰的蛋白质发生反应，而不与未修饰的蛋白质发生交叉反应。所有抗体对BPDE-I-四醇的敏感性都高于对BPDE-I修饰蛋白质的敏感性。它们与BPDE-I-脱氧鸟苷、BPDE-I-DNA和BPDE-II-兔血清白蛋白发生交叉反应。由于抗体对四醇具有高敏感性，因此开发了一种免疫测定法，用于基于从修饰样品中释放出的四醇来定量DNA和蛋白质加合物。使用放射性标记的BPDE-I修饰的DNA和蛋白质对该方法进行了验证。
