Xu Yu, Wang Wei, Wang He, Tian Yinping, Yue Zhengfu, Li Cheng, Wang Yuefeng, Zhang Jing, Zhang Ruifu
Key Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.
Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2024 Oct 17;15:1405527. doi: 10.3389/fpls.2024.1405527. eCollection 2024.
Efficient utilization of agricultural wastes and reduction of chemical fertilizer inputs are crucial for sustainable development of agriculture. Plant growth promoting rhizobacteria (PGPR) are widely used as biofertilizers to partially replace chemical fertilizers in agricultural production. The functional performance of PGPR strains is closely related to their root colonization capacity. Some organic acids from root exudates can recruit PGPR to colonize the root. In this study, agricultural organic wastes such as mushroom bran and tobacco waste materials were used to produce organic acids through the hypoxic hydrolysis process. The hydrolysis conditions were optimized to maximize the production of a mixture of complex organic acids from the hypoxic hydrolysis of these materials, employing both single-factor and orthogonal experimental methods. The diluted hydrolysates were tested for their effects on the rhizosphere colonization of the PGPR strain SQR9 using fluorogenic quantitative PCR in greenhouse pot experiments. The results demonstrated that hypoxic hydrolysates from tobacco waste and mushroom bran significantly enhanced the colonization of SQR9 in the maize rhizosphere. Specifically, a 2000-fold dilution of tobacco waste hydrolysate yielded the most effective result, while a 5000-fold dilution of mushroom bran hydrolysate provided the best outcome. All treatments combining these hydrolysates with SQR9 significantly increased maize stem dry weight, indicating that with appropriate treatment, such as anaerobic fermentation, these agricultural organic wastes can serve as synergistic agents of microbial fertilizers, contributing to agricultural sustainability.
高效利用农业废弃物并减少化肥投入对农业可持续发展至关重要。植物促生根际细菌（PGPR）作为生物肥料被广泛用于农业生产中以部分替代化肥。PGPR菌株的功能表现与其根部定殖能力密切相关。根系分泌物中的一些有机酸可招募PGPR定殖于根部。在本研究中，利用蘑菇渣和烟草废弃物等农业有机废弃物通过缺氧水解过程生产有机酸。采用单因素和正交试验方法优化水解条件，以使这些材料缺氧水解产生的复合有机酸混合物产量最大化。在温室盆栽试验中，使用荧光定量PCR检测稀释后的水解产物对PGPR菌株SQR9根际定殖的影响。结果表明，烟草废弃物和蘑菇渣的缺氧水解产物显著增强了SQR9在玉米根际的定殖。具体而言，烟草废弃物水解产物2000倍稀释效果最佳，而蘑菇渣水解产物5000倍稀释效果最佳。所有将这些水解产物与SQR9结合的处理均显著增加了玉米茎干重，表明经过适当处理，如厌氧发酵，这些农业有机废弃物可作为微生物肥料的增效剂，促进农业可持续发展。
