Chen Jan-Yow, Li Chien-Yu, Mong Mei-Chin, Yin Mei-Chin
Department of Internal Medicine, China Medical University Hospital, Taichung 404328, Taiwan.
Department of Neurosurgery, Asia University Hospital, Taichung 413305, Taiwan.
Asian Biomed (Res Rev News). 2024 Oct 31;18(5):224-235. doi: 10.2478/abm-2024-0030. eCollection 2024 Oct.
The health benefits of coixol, an active compound of adlay seed, have attracted certain attention. Adlay seed is often adopted in traditional Chinese medicine for the treatment of various inflammatory disorders. Thus, it is hypothesized that coixol could protect neuronal cells.
The preventive effects of coixol against Abeta-induced damage in nerve growth factor-differentiated PC12 cells were explored.
Differentiated PC12 cells were treated with coixol at 0.125 μM, 0.25 μM, 0.5 μM, 1 μM, and 2 μM for 48 h. Then, cells were further exposed to Abeta at 20 μM for 24 h.
Coixol treatments at 0.25-2 μM exhibited antiapoptotic effect via increasing Bcl-2 mRNA expression, mitochondrial membrane potential, and Na-K ATPase activity as well as decreasing Bax mRNA expression, caspase-3 activity, and intracellular Ca release. In addition, coixol treatments at 0.25-2 μM alleviated oxidative and inflammatory responses via lowering reactive oxygen species level, increasing glutathione content, promoting the activity of glutathione peroxidase, glutathione reductase, and catalase, decreasing the generation of tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and prostaglandin E. Furthermore, coixol treatments at 0.25-2 μM diminished intracellular Ca release, and restricted nuclear factor kappa B-binding activity and phosphorylation of p65 and p38. Coixol treatments at 0.5-2 μM increased protein generation of nuclear factor E2-related factor 2, and limited protein production of inducible nitric oxide synthase and receptor of advanced glycation end product.
Our novel findings suggested that coixol was a compelling agent against beta-amyloid peptide-induced neurotoxicity.
薏苡仁种子的活性化合物薏苡仁醇的健康益处已引起一定关注。薏苡仁种子常用于传统中药中治疗各种炎症性疾病。因此，推测薏苡仁醇可能保护神经元细胞。
探讨薏苡仁醇对神经生长因子分化的PC12细胞中β淀粉样蛋白（Aβ）诱导损伤的预防作用。
将分化的PC12细胞分别用0.125μM、0.25μM、0.5μM、1μM和2μM的薏苡仁醇处理48小时。然后，细胞再用20μM的Aβ处理24小时。
0.25 - 2μM的薏苡仁醇处理通过增加Bcl - 2 mRNA表达、线粒体膜电位和钠钾ATP酶活性，以及降低Bax mRNA表达、半胱天冬酶 - 3活性和细胞内钙释放，表现出抗凋亡作用。此外，0.25 - 2μM的薏苡仁醇处理通过降低活性氧水平、增加谷胱甘肽含量、促进谷胱甘肽过氧化物酶、谷胱甘肽还原酶和过氧化氢酶的活性，减少肿瘤坏死因子 - α、白细胞介素（IL）-1β、IL - 6和前列腺素E的产生，减轻氧化和炎症反应。此外，0.25 - 2μM的薏苡仁醇处理减少细胞内钙释放，并限制核因子κB结合活性以及p65和p38的磷酸化。0.5 - 2μM的薏苡仁醇处理增加核因子E2相关因子2的蛋白质生成，并限制诱导型一氧化氮合酶和晚期糖基化终产物受体的蛋白质产生。
我们的新发现表明，薏苡仁醇是一种对抗β淀粉样肽诱导的神经毒性的有效药物。
