Department of Orthopaedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, PR China.
Department of Surgery of Chinese Medicine, Jiangxi University of Chinese Medicine, Jiangxi 330004, PR China.
Carbohydr Polym. 2025 Jan 1;347:122723. doi: 10.1016/j.carbpol.2024.122723. Epub 2024 Sep 5.
Osteomyelitis is a refractory disease of orthopedics, part of which is caused by medical implants. The main difficulties in treatment are the barrier effect after the formation of bacterial biofilm, and the difficulty in achieving sustained antibiotic intervention. In view of this situation, we studied a hydrogel coating that can release CaCl and vancomycin in pH-responsive manner. We used nano-TiO to modify Chitosan/ Gelatin/Aldehyde Hyaluronic Acid (CS/Gel/AHA) hydrogel, and combined with the dip-coating technique, prepared a coating with good mechanical strength. The hydrogel-loaded zeolitic imidazolate framework (ZIF) decomposes under acidic conditions, and the released Ca act on the bacterial Bap protein to inhibit the formation of biofilm, and the released vancomycin kills free bacteria. The antibacterial coating achieved good bactericidal effect in both in vitro experiments and rat subcutaneous implant model. These results not only provide a new way to enhance the strength of hydrogels to prepare coatings, but also utilize a new approach to responsively inhibit the formation of biofilms, showing the promising application prospects of the coating in antibacterial treatment of medical implants.
骨髓炎是骨科的一种难治性疾病，其中一部分是由医用植入物引起的。治疗的主要难点在于细菌生物膜形成后的屏障作用，以及难以实现持续的抗生素干预。针对这种情况，我们研究了一种水凝胶涂层，该涂层可以在 pH 响应的情况下释放 CaCl 和万古霉素。我们使用纳米 TiO2 对壳聚糖/明胶/醛基透明质酸（CS/Gel/AHA）水凝胶进行修饰，并结合浸涂技术，制备了具有良好机械强度的涂层。水凝胶负载的沸石咪唑酯骨架（ZIF）在酸性条件下分解，释放的 Ca 作用于细菌 Bap 蛋白，抑制生物膜的形成，释放的万古霉素杀死游离细菌。该抗菌涂层在体外实验和大鼠皮下植入模型中均取得了良好的杀菌效果。这些结果不仅为增强水凝胶强度制备涂层提供了新方法，还利用响应性抑制生物膜形成的新方法，展示了该涂层在医用植入物抗菌治疗中的应用前景。
