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顺式 9，反式 11-共轭亚油酸对奶牛乳腺上皮细胞氧化应激和炎症损伤的保护作用。

Protection effect of cis 9, trans 11-conjugated linoleic acid on oxidative stress and inflammatory damage in bovine mammary epithelial cells.

机构信息

College of Animal Science and Technology, Inner Mongolia Minzu University, Tong Liao, People's Republic of China.

College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tong Liao, People's Republic of China.

出版信息

Sci Rep. 2024 Nov 1;14(1):26295. doi: 10.1038/s41598-024-77711-0.

DOI:10.1038/s41598-024-77711-0
PMID:39487250
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11530692/
Abstract

The present study was conducted to observe the protective effects of c9, t11- conjugated linoleic acid (CLA) on oxidative stress and inflammation in bovine mammary epithelial cells (BMECs) exposed to HO. The BMECs were treated with different concentrations of HO for 8 h, 600 µmol/L was determined to be the damage concentration. Using different concentrations of c9, t11-CLA to process BMECs for 24 h, 50 and 100 µmol/L were determined to be the effective concentrations for subsequent analyses. Thus, four BMEC groups were established: Control group; HO group; 50 µmol/L c9, t11-CLA + HO group; 100 µmol/L c9, t11-CLA + HO group. We observed that the HO group exhibited significantly lower total antioxidant activity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and significantly higher secretions of malondialdehyde (MDA), interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α and expressions of IL-1β, IL-6, and IL-8 than the control group (p < 0.05). Pretreatment with c9, t11-CLA enhanced SOD, CAT, and GPx activities and SOD mRNA expression and repressed IL-6 and IL-8 secretion and expression in HO-treated BMECs (p < 0.05). In conclusion, c9, t11-CLA treatment efficiently enhanced antioxidant capacity and decreased inflammation induced by HO in BMECs.

摘要

本研究旨在观察 c9，t11-共轭亚油酸（CLA）对 HO 诱导的奶牛乳腺上皮细胞（BMEC）氧化应激和炎症的保护作用。用不同浓度的 HO 处理 BMEC 8 h，确定 600 μmol/L 为损伤浓度。用不同浓度的 c9，t11-CLA 处理 BMEC 24 h，确定 50 和 100 μmol/L 为后续分析的有效浓度。因此，建立了四个 BMEC 组：对照组；HO 组；50 μmol/L c9，t11-CLA+HO 组；100 μmol/L c9，t11-CLA+HO 组。结果发现，HO 组的总抗氧化活性（T-AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性显著降低，丙二醛（MDA）、白细胞介素（IL）-1β、IL-6、IL-8 和肿瘤坏死因子（TNF）-α的分泌显著升高，IL-1β、IL-6 和 IL-8 的表达也显著升高（p<0.05）。用 c9，t11-CLA 预处理可增强 SOD、CAT 和 GPx 的活性和 SOD mRNA 的表达，抑制 HO 处理的 BMEC 中 IL-6 和 IL-8 的分泌和表达（p<0.05）。综上所述，c9，t11-CLA 处理可有效增强 BMEC 对 HO 诱导的抗氧化能力，降低炎症反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0b84/11530692/3224af40f153/41598_2024_77711_Fig7_HTML.jpg
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