非靶向代谢组学分析小细胞外囊泡揭示三阴性乳腺癌潜在的新型生物标志物。

Untargeted metabolomic profiling of small extracellular vesicles reveals potential new biomarkers for triple negative breast cancer.

机构信息

Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Canada.

John L. Holmes Biological Mass Spectrometry Facility, University of Ottawa, Ottawa, Canada.

出版信息

Metabolomics. 2024 Nov 2;20(6):123. doi: 10.1007/s11306-024-02191-2.

DOI:10.1007/s11306-024-02191-2

PMID:39487276

Abstract

INTRODUCTION

Breast Cancer (BC) is one of the most diagnosed malignancies among women and the second leading cause of cancer related death in North America. Triple Negative BC (TNBC), one of the most severe subtypes of BC, is extremely aggressive and has a higher chance of occurrence in women under 50 years of age. Due to a lack of regular mammographic testing in women under 50, many individuals with TNBC are diagnosed late which can decrease their survival rate. Currently, liquid biopsy is being investigated as a potentially less-invasive alternative to traditional breast tissue biopsy, but this approach is not completely reliable. Blood contains extracellular vesicles (EVs), which carry biomolecular cargo and play a role in BC progression and metastasis. Examination of small EVs could potentially yield metabolite biomarkers for early BC diagnosis.

OBJECTIVE

We aim to study metabolites in small EVs to find biomarkers for BC diagnosis.

METHODS

In this work, an untargeted nano-LC MS/MS metabolomics approach was used to analyze metabolites from small EVs derived from metastatic MDA-MB-231 and compare it with a non-cancerous MCF10A cell line.

RESULTS

Two metabolites, LysoPC 22:6/0:0 and N-acetyl-L-Phenylalanine, unique to sEVs of MDA-MB-231, were identified, validated, and proposed as potential BC biomarkers.

CONCLUSION

Metabolites from sEVs may be used for BC diagnosis.

摘要

简介

乳腺癌（BC）是女性中最常见的恶性肿瘤之一，也是北美癌症相关死亡的第二大主要原因。三阴性乳腺癌（TNBC）是 BC 最严重的亚型之一，极具侵袭性，且在 50 岁以下女性中的发病率更高。由于 50 岁以下女性常规乳房 X 光检查的缺乏，许多 TNBC 患者被诊断得较晚，这可能降低了她们的存活率。目前，液体活检正被研究作为一种潜在的、侵入性更小的传统乳房组织活检替代方法，但这种方法并非完全可靠。血液中含有细胞外囊泡（EVs），其携带生物分子货物，在 BC 的进展和转移中发挥作用。对小 EV 进行检查可能会产生用于早期 BC 诊断的代谢物生物标志物。