Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh, 11355, Saudi Arabia.
Department of Chemistry, College of Science Al Zulfi, Majmaah University, Zulfi, Saudi Arabia.
Sci Rep. 2024 Nov 1;14(1):26300. doi: 10.1038/s41598-024-78039-5.
Monitoring the concentration of Fludarabine phosphate, a standard chemotherapeutic drug widely used in cancer treatment, is vital for ensuring the drug's safety and effectiveness, tailoring treatments to individual needs, and consequently improving overall patient outcomes. Regarding the limitations of conventional techniques in terms of complexity, large time measurements, and a high cost, there is an urgent need to develop simple, rapid, and cost-effective devices. In this paper, we report the design of an aptasensor for the specific and selective detection of Fludarabine. Systematic evolution of ligands by exponential enrichment (SELEX) protocol was performed to select a specific aptamer for Fludarabine. Eleven rounds were carried out, and nine sequences were selected. Based on the dissociation constant (Kd), Ful-3, exhibiting the highest affinity (18.86 nM), was chosen and integrated into a simple electrochemical aptasensing platform for Fludarabine detection. Electrochemical results demonstrated good performance of the selected aptamer in detecting Fludarabine within the analytical range of 1 to 150 pg/mL and with LOD and LOQ in the order of 0.11 pg/mL (0.31 fM) and 0.39 pg/mL (1.06 fM), respectively. The developed platform also showed good selectivity against different analogous molecules and high applicability in serum-spiked samples, with recovery percentages ranging from 96.81 to 99.04%. Considering the encouraging results, this research presents an excellent alternative in terms of simplicity, stability, ease of use, reduction of sample volume, and low cost.
监测氟达拉滨磷酸盐（一种广泛用于癌症治疗的标准化疗药物）的浓度对于确保药物的安全性和有效性、针对个体需求定制治疗方案以及提高整体患者预后至关重要。鉴于传统技术在复杂性、大时间测量和高成本方面的局限性，迫切需要开发简单、快速且具有成本效益的设备。本文报道了一种用于氟达拉滨特异性和选择性检测的适体传感器的设计。通过指数富集的配体系统进化（SELEX）方案选择了氟达拉滨的特异性适体。进行了 11 轮筛选，选择了 9 个序列。基于解离常数（Kd），具有最高亲和力（18.86 nM）的 Ful-3 被选择并整合到用于氟达拉滨检测的简单电化学适体传感平台中。电化学结果表明，所选适体在检测 1 至 150 pg/mL 范围内的氟达拉滨时具有良好的性能，LOD 和 LOQ 分别为 0.11 pg/mL（0.31 fM）和 0.39 pg/mL（1.06 fM）。该开发的平台还表现出对不同类似分子的良好选择性和在血清加标样品中的高适用性，回收率百分比范围为 96.81%至 99.04%。考虑到令人鼓舞的结果，本研究在简单性、稳定性、易用性、减少样品体积和低成本方面提供了一个极好的选择。
