UMRT INRAE 1158 BioEcoAgro, Laboratoire BIOPI, University of Picardie Jules Verne, Amiens, 80000, France.
, AgroStation, Rue de la Station, Aspach-le-Bas, 68700, France.
Metabolomics. 2024 Nov 2;20(6):122. doi: 10.1007/s11306-024-02192-1.
Since the use of a bio stimulant should provide a response to a problem that depends on the production system implemented (crops, plant model, soil, climate, the farmer's practices…), the agricultural sector is facing concomitant challenges of choosing the best bio stimulant that suits their needs. Thus, understanding bio stimulant-plant interactions, at molecular level, using metabolomics approaches is a prerequisite, for the development of a bio stimulant, leading to an effective exploration and application of formulations in agriculture. AGRO-K®, is commercialized as a plant-based bio stimulant that improve vigor and enhance resistance to lodging in cereal crops. A recent previous untargeted metabolomics study has demonstrated the ability of this bio stimulant to improve wheat resistance to lodging, in real open-field conditions. However, the reproducibility of the impact of this bio stimulant in other filed crops is not yet investigated.
Therefore, the present study aimed to assess the changes in primary and secondary metabolites in the roots, stems, and leaves of fiber flax (Linum usitatissimum L), treated with the bio stimulant, using NMR and LC-MS-based untargeted metabolomics approach.
In addition to the previous result conducted in wheat, the present analysis seemed to show that this bio stimulant led to a similar pathway enhancement in flax. The pathways which seem to be reproducibly impacted are hydroxycinnamic acid amides (HCAAs), phenylpropanoids and flavonoids. Impacting these pathways enhance root growth and elongation and cell wall lignification, which can aid in preventing crop lodging. These results confirm that HCAAs, flavonoids, and phenylpropanoids could serve as signatory biomarkers of the impact of AGRO-K® on improving lodging resistance across various plant species.
由于生物刺激剂的使用应该针对依赖于所实施的生产系统（作物、植物模型、土壤、气候、农民的实践……）的问题提供响应，因此农业部门面临着同时选择最适合其需求的最佳生物刺激剂的挑战。因此，使用代谢组学方法理解生物刺激剂-植物相互作用，在分子水平上，是开发生物刺激剂的前提，从而有效地探索和应用农业中的制剂。AGRO-K®作为一种基于植物的生物刺激剂，可提高谷物的活力并增强抗倒伏能力。最近的一项非靶向代谢组学研究表明，这种生物刺激剂有能力提高小麦的抗倒伏能力，在真实的田间条件下。然而，这种生物刺激剂在其他田间作物中的影响的重现性尚未得到研究。
因此，本研究旨在使用基于 NMR 和 LC-MS 的非靶向代谢组学方法，评估纤维亚麻（Linum usitatissimum L）的根、茎和叶中初级和次生代谢物的变化，用生物刺激剂处理。
除了之前在小麦上进行的研究结果外，本分析似乎表明，这种生物刺激剂在亚麻中导致了类似的途径增强。似乎可重现影响的途径是羟基肉桂酰胺（HCAAs）、苯丙素和类黄酮。影响这些途径可以增强根系生长和伸长以及细胞壁木质化，这有助于防止作物倒伏。这些结果证实 HCAAs、类黄酮和苯丙素可以作为 AGRO-K® 提高各种植物物种抗倒伏能力的影响的标志生物标志物。
