循环 microRNA-409-5p 和 USP7 与急性心肌梗死患者的左心室重构相关。

Circulating microRNA-409-5p and USP7 are associated with left ventricular remodeling in patients with acute myocardial infarction.

机构信息

Department of Cardiology, Yan'an Hospital of Kunming City, Yan'an Hospital Affiliated To Kunming Medical University, Kunming, 650051, China.

Kunming Cardiovascular Interventional Imaging Institute, Kunming, China.

出版信息

BMC Cardiovasc Disord. 2024 Nov 2;24(1):615. doi: 10.1186/s12872-024-04299-8.

DOI:10.1186/s12872-024-04299-8

PMID:39488705

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11531163/

Abstract

BACKGROUND

Our previous study demonstrated that microRNA-409-5p (miR-409-5p) and its target ubiquitin-specific protease 7 (USP7) were involved in hypoxia-induced cardiomyocyte injury and ischemic left ventricular remodeling (LVR) in rats. This study aimed to probe into the relationship between plasma miR-409-5p and USP7 levels and LVR and dysfunction in patients with acute myocardial infarction (AMI).

METHODS

Sixty patients with acute myocardial infarction (AMI) and 60 cases with chronic coronary syndrome (CCS) were enrolled. The clinical characteristics, echocardiographic/serum parameters of LVR, and circulating miR-409-5p and USP7 mRNA levels between the two groups and between admission and 6 weeks after discharge were compared. The correlations between circulating miR-409-5p/USP7 levels and serum/echocardiographic parameters were analyzed.

RESULTS

The demographic characteristics of the AMI group and CCS group were comparable. Patients with AMI admitted to the study displayed significantly higher levels of plasma N-terminal pro-brain natriuretic peptide (NT-proBNP) and growth stimulation expressed gene 2 (ST2), along with a greater left ventricular end-systolic volume (LVESV). Conversely, their left ventricular ejection fraction (LVEF), global longitudinal strain (GLS), and global radial strain (GRS) were significantly lower compared to patients with CCS. These changes were normalized 6 weeks after discharge. Circulating miR-409-5p levels at admission were significantly decreased while circulating USP7 mRNA expression levels were significantly increased in patients with AMI compared with those with CCS (both P < 0.01). However, these changes were restored 6 weeks after discharge (both P < 0.01). Moreover, circulating miR-409-5p and USP7 mRNA levels showed varying correlations with GLS, GRS, LVESV, LVEF, and NT-proBNP in patients with AMI but not in those with CCS. Additionally, circulating miR-409-5p and USP7 levels predicted the incidence of LVR and major adverse cardiovascular events (MACE) after AMI.

CONCLUSION

Serum miR-409-5p and USP7 may influence the occurrence and evolution of LVR and left ventricular dysfunction after AMI.

摘要

背景

我们之前的研究表明，微小 RNA-409-5p（miR-409-5p）及其靶标泛素特异性蛋白酶 7（USP7）参与了大鼠缺氧诱导的心肌细胞损伤和缺血性左心室重构（LVR）。本研究旨在探讨急性心肌梗死（AMI）患者血浆 miR-409-5p 和 USP7 水平与 LVR 和心功能障碍的关系。

方法

纳入 60 例急性心肌梗死（AMI）患者和 60 例慢性冠状动脉综合征（CCS）患者。比较两组患者入院时及出院后 6 周的临床特征、LVR 超声心动图/血清学参数、循环 miR-409-5p 和 USP7mRNA 水平，并分析循环 miR-409-5p/USP7 水平与血清/超声心动图参数的相关性。

结果

AMI 组和 CCS 组患者的人口统计学特征相似。研究纳入的 AMI 患者的血浆 N 末端脑利钠肽前体（NT-proBNP）和生长刺激表达基因 2（ST2）水平显著升高，左心室收缩末期容积（LVESV）增大，而左心室射血分数（LVEF）、整体纵向应变（GLS）和整体径向应变（GRS）则显著降低，与 CCS 患者相比。这些变化在出院后 6 周时恢复正常。与 CCS 患者相比，AMI 患者入院时循环 miR-409-5p 水平显著降低，而循环 USP7mRNA 表达水平显著升高（均 P<0.01），但在出院后 6 周时恢复正常（均 P<0.01）。此外，在 AMI 患者中，循环 miR-409-5p 和 USP7mRNA 水平与 GLS、GRS、LVESV、LVEF 和 NT-proBNP 呈不同程度的相关性，但在 CCS 患者中无相关性。此外，循环 miR-409-5p 和 USP7 水平可预测 AMI 后 LVR 和主要不良心血管事件（MACE）的发生。