Li Xuemei, Xiao Xi, Zhang Yiheng, Long Ruimin, Kankala Ranjith Kumar, Wang Shibin, Liu Yuangang
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China; Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Biochemical Technology, Xiamen 361021, China.
Int J Biol Macromol. 2024 Dec;282(Pt 5):137185. doi: 10.1016/j.ijbiomac.2024.137185. Epub 2024 Nov 1.
Diabetic wound healing has become one of the major clinical burdens due to uncontrolled bacterial growth and an increase in the risk of various microbial infections. Despite excellent antioxidant properties, the poor aqueous solubility of resveratrol (RES) hampers its applicability. In this study, we proposed a novel multifunctional microneedle patch loaded with RES-encapsulated polymeric micelles. Resveratrol micelles (RES MC) were loaded in the microneedle tip, while the base part was coated with the antibiotic gentamicin (GEN) to promote wound healing. The microneedle tip composed of sodium hyaluronate (HA) could effectively deliver the anti-inflammatory and antioxidant RES MC. Furthermore, the base of the microneedle patch composed of polyvinyl alcohol (PVA) offered excellent flexibility, releasing GEN and providing resistance to bacterial contamination, thereby further promoting wound repair. In vitro antibacterial experiments indicated that the bactericidal rate reached 99 %. Further, the wound healing rate was recorded as 86.05 % on the 11th day of diabetes wound treatment. Together, the multifunctional microneedle patch with excellent biocompatibility exhibited anti-inflammatory, antioxidant, and antibacterial effects on the wound healing process, potentiating its efficacy in the treatment of diabetic wounds.
由于细菌生长失控以及各种微生物感染风险增加，糖尿病伤口愈合已成为主要的临床负担之一。尽管白藜芦醇（RES）具有出色的抗氧化特性，但其水溶性差限制了其应用。在本研究中，我们提出了一种新型的多功能微针贴片，其装载有包裹RES的聚合物胶束。白藜芦醇胶束（RES MC）装载在微针尖端，而基部涂有抗生素庆大霉素（GEN）以促进伤口愈合。由透明质酸钠（HA）组成的微针尖端可有效递送具有抗炎和抗氧化作用的RES MC。此外，由聚乙烯醇（PVA）组成的微针贴片基部具有出色的柔韧性，可释放GEN并提供抗细菌污染能力，从而进一步促进伤口修复。体外抗菌实验表明杀菌率达到99%。此外，在糖尿病伤口治疗的第11天，伤口愈合率记录为86.05%。总之，具有出色生物相容性的多功能微针贴片在伤口愈合过程中表现出抗炎、抗氧化和抗菌作用，增强了其在糖尿病伤口治疗中的疗效。
