Zhou Junjie, Gao Wenhua, Wu Jinglin, Xiang Zhouyang, Zeng Jinsong, Wang Bin, Xu Jun
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Int J Biol Macromol. 2024 Dec;282(Pt 3):137115. doi: 10.1016/j.ijbiomac.2024.137115. Epub 2024 Nov 3.
Cellulose nanofibrils (CNFs) can serve as an efficient surface enhanced Raman scattering (SERS) platform for in situ detection of trace targets. In this study, a highly reproducible SERS platform based on TEMPO-oxidized CNFs (T-CNFs) was fabricated by the ion-exchange. Self-assembly of silver nanoparticles (AgNPs) was accomplished in only 120 s. The abundant carboxylate groups and good hydrophilicity of T-CNFs facilitated uniform and dense loading of AgNPs over the surface area. The obtained SERS substrate greatly enhanced the Raman signal of different pesticides, and the detection limits of thiram and thiabendazole were 5.81 × 10 M and 9.63 × 10 M, respectively. SERS substrate could produce homogeneous Raman-enhanced signals (relative standard deviation (RSD) = 6.59 %). In addition, due to the good flexibility, SERS substrate could collect and detect pesticide residues from the surface of apples. The intensities of Raman characteristic peak at 1384 cm showed a good linear relationship with the analyte concentrations (0.96 ng/cm-9600 ng/cm). The constructed SERS substrate provided a theoretical basis for the preliminary rapid screening of hazardous chemical residues in food, which was of great value for the SERS technique to become a routine on-site analysis method for pesticide residues.
纤维素纳米纤维（CNFs）可作为一种高效的表面增强拉曼散射（SERS）平台，用于原位检测痕量目标物。在本研究中，通过离子交换制备了一种基于2,2,6,6-四甲基哌啶-1-氧自由基（TEMPO）氧化的CNFs（T-CNFs）的高重现性SERS平台。银纳米颗粒（AgNPs）的自组装仅需120秒即可完成。T-CNFs丰富的羧基基团和良好的亲水性有助于AgNPs在其表面均匀且密集地负载。所获得的SERS基底极大地增强了不同农药的拉曼信号，福美双和噻菌灵的检测限分别为5.81×10⁻⁸M和9.63×10⁻⁸M。SERS基底能够产生均匀的拉曼增强信号（相对标准偏差（RSD）=6.59%）。此外，由于具有良好的柔韧性，SERS基底能够从苹果表面收集并检测农药残留。1384 cm⁻¹处拉曼特征峰的强度与分析物浓度（0.96 ng/cm² - 9600 ng/cm²）呈现出良好的线性关系。所构建的SERS基底为食品中有害化学残留的初步快速筛查提供了理论依据，这对于SERS技术成为农药残留的常规现场分析方法具有重要价值。
