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自体诱导物 2 通过调节芽孢形成信号来缓解土壤胁迫诱导的韦氏芽孢杆菌休眠。

Autoinducer-2 relieves soil stress-induced dormancy of Bacillus velezensis by modulating sporulation signaling.

机构信息

National Engineering Research Center of Navel Orange, Gannan Normal University, Ganzhou, 341000, PR China.

State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China, the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, Beijing, PR China.

出版信息

NPJ Biofilms Microbiomes. 2024 Nov 3;10(1):117. doi: 10.1038/s41522-024-00594-6.

DOI:10.1038/s41522-024-00594-6
PMID:39489748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11532509/
Abstract

The collective behavior of bacteria is regulated by quorum sensing (QS). Autoinducer-2 (AI-2) is a common QS signal that regulates the behavior of both Gram-positive and Gram-negative bacteria. Despite the plethora of processes described to be influenced by AI-2 in diverse Gram-negative bacteria, the AI-2-regulated processes in Bacilli are relatively unexplored. Here, we describe a novel function for AI-2 in Bacillus velezensis SQR9 related to the sporulation. AI-2 inhibited the initiation of sporulation through the phosphatase RapC and the DNA binding regulator ComA. Using biochemistry experiments, we demonstrated that AI-2 interacts with RapC to stimulate its binding to ComA, which leads to an inactive ComA and subsequently a sporulation inhibition. The AI-2 molecule could be shared across species for inhibiting Bacillus sporulation and it also plays the same role in different soil conditions. Our study revealed a novel function and regulatory mechanism of AI-2 in inhibiting sporulation in Bacilli.

摘要

群体感应（QS）调控细菌的集体行为。双（酰）基高丝氨酸内酯（AI-2）是一种常见的 QS 信号，可调节革兰氏阳性菌和革兰氏阴性菌的行为。尽管有大量的研究描述了 AI-2 在不同的革兰氏阴性菌中对各种过程的影响，但芽孢杆菌中 AI-2 调控的过程相对较少。在这里，我们描述了一种新型的 AI-2 功能，它与芽孢杆菌 SQR9 的孢子形成有关。AI-2 通过磷酸酶 RapC 和 DNA 结合调控因子 ComA 抑制孢子形成的起始。通过生化实验，我们证明 AI-2 与 RapC 相互作用，刺激其与 ComA 结合，导致 ComA 失活，进而抑制孢子形成。AI-2 分子可以在不同物种之间共享，以抑制芽孢杆菌的孢子形成，并且在不同的土壤条件下也发挥相同的作用。我们的研究揭示了 AI-2 在抑制芽孢杆菌孢子形成中的新型功能和调控机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/11532509/3a15dd0b53c4/41522_2024_594_Fig7_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9d8/11532509/3a15dd0b53c4/41522_2024_594_Fig7_HTML.jpg
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Fatty Acid Synthesis Knockdown Promotes Biofilm Wrinkling and Inhibits Sporulation in Bacillus subtilis.脂肪酸合成敲低促进枯草芽孢杆菌生物膜起皱并抑制孢子形成。
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