State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
College of Animal Science, Zhejiang A&F University, Hangzhou, China.
Front Immunol. 2022 Feb 10;13:844102. doi: 10.3389/fimmu.2022.844102. eCollection 2022.
The ileum is mainly responsible for food absorption and nutrients transportation. The microbes in its intestinal lumen play an essential role in the growth and health of the host. However, it is still unknown how the ileal microbes affect the body weight of the host. In this study, we used Muscovy ducks as an animal model to investigate the relationship between the ileal microbes and body weight and further explore the potential mechanism. The ileum tissue and ileal contents of 200 Muscovy ducks were collected for mRNA extraction and real-time quantitative PCR, as well as DNA separation and 16S rRNA gene sequencing. With body weight being ranked, the bottom 20% (n = 40) and top 20% (n = 40) were set as the low and high groups, respectively. Our results showed that in the ileum of Muscovy ducks, the Bacteroides, Firmicutes, and Proteobacteria were the predominant phyla with the 10 most abundant genera, namely , , , , , , , , , and We identified , , , , , and were closely correlated to the growth of Muscovy ducks. and were negatively related to body weight (BW), while , , , and were positively associated with BW. In addition, we found that the relative expression levels of tight junction proteins (, , and ) in the high group showed an upward trend, although this trend was not significant ( > 0.05). The expression of pro-inflammatory factors (, and ) decreased in the high group, while the anti-inflammatory factor increased. Of course, except , these differences were not significant ( > 0.05). Finally, the correlation analysis showed that was significantly positively correlated with ( < 0.05). These findings may provide fundamental data for the development of next-generation probiotics and assist the development of strategies for changing the gut microbiota to promote the growth performance in the duck industry.
回肠主要负责食物吸收和营养物质运输。其肠腔中的微生物在宿主的生长和健康中起着至关重要的作用。然而，回肠微生物如何影响宿主的体重仍不清楚。在这项研究中，我们使用麝香鸭作为动物模型，研究回肠微生物与体重之间的关系，并进一步探讨其潜在机制。收集 200 只麝香鸭的回肠组织和回肠内容物进行 mRNA 提取和实时定量 PCR，以及 DNA 分离和 16S rRNA 基因测序。根据体重进行排序，将最低的 20%（n=40）和最高的 20%（n=40）分别设为低体重组和高体重组。我们的结果表明，在麝香鸭的回肠中，拟杆菌门、厚壁菌门和变形菌门是主要的门，其中 10 个最丰富的属分别为 、 、 、 、 、 、 、 、和 。我们鉴定出 、 、 、 、和 与麝香鸭的生长密切相关。和 与 BW 呈负相关，而 、 、 和 与 BW 呈正相关。此外，我们发现高体重组中紧密连接蛋白（ 、 、 和 ）的相对表达水平呈上升趋势，尽管这种趋势不显著（ > 0.05）。高体重组中促炎因子（ 、 和 ）的表达降低，而抗炎因子 增加。当然，除了 之外，这些差异并不显著（ > 0.05）。最后，相关性分析表明 与 呈显著正相关（ < 0.05）。这些发现可能为开发下一代益生菌提供基础数据，并有助于开发改变肠道微生物群以促进鸭业生长性能的策略。
