State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, China.
Bioresour Technol. 2025 Jan;416:131714. doi: 10.1016/j.biortech.2024.131714. Epub 2024 Oct 28.
Syngas can be efficiently converted to acetate by Moorella thermoacetica under anaerobic conditions, which is environmentally friendly. Coupled with acetate production from syngas, using acetate to synthesize value-added compounds such as short-chain carboxylic acids (SCCAs) becomes a negative-carbon process. Escherichia coli is engineered to utilize acetate as the sole carbon source to produce SCCAs. By knocking out some acetyltransferase genes, introducing exogenous pathway and additional cofactor engineering, the strains can synthesize 3.79 g/L of 3-hydroxypropionic acid (3-HP), 1.83 g/L of (R)-3-hydroxybutyric acid (R-3HB), and 2.31 g/L of butyrate. We used M. thermoacetica to produce acetate from syngas. Subsequently, all engineered E. coli strains were able to produce SCCAs from syngas-derived acetate. The titers of 3-HP, R-3HB, and butyrate are 3.75, 1.68, and 2.04 g/L, with carbon sequestration rates of 51.1, 26.3, and 38.1 %. This coupled bioprocess has great potential for producing a range of other value-added chemicals from syngas.
在厌氧条件下，热醋穆尔氏菌（Moorella thermoacetica）可以有效地将合成气转化为醋酸盐，这是环保的。结合合成气生产醋酸盐，利用醋酸盐合成附加值化合物，如短链羧酸（SCCAs），成为一种负碳过程。大肠杆菌被设计为利用醋酸盐作为唯一碳源来生产 SCCAs。通过敲除一些乙酰基转移酶基因，引入外源途径和额外的辅因子工程，可以使菌株合成 3.79 g/L 的 3-羟基丙酸（3-HP）、1.83 g/L 的（R）-3-羟基丁酸（R-3HB）和 2.31 g/L 的丁酸。我们使用热醋穆尔氏菌（Moorella thermoacetica）从合成气中生产醋酸盐。随后，所有工程大肠杆菌菌株都能够从合成气衍生的醋酸盐中生产 SCCAs。3-HP、R-3HB 和丁酸的产量分别为 3.75、1.68 和 2.04 g/L，碳固存率分别为 51.1%、26.3%和 38.1%。这种耦合生物过程具有从合成气生产一系列其他有价值化学品的巨大潜力。
