Chaweewannakorn Chayanit, Aye Khin The Nu, Ferreira Joao N
Center of Excellence and Innovation for Oral Health and Health Longevity, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand; Department of Occlusion, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
Center of Excellence and Innovation for Oral Health and Health Longevity, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
SLAS Discov. 2024 Dec;29(8):100190. doi: 10.1016/j.slasd.2024.100190. Epub 2024 Oct 28.
Over the past decade, there has been a rapid development in the use of magnetic three dimensional (3D) based cell culture systems. Concerning the skeletal muscle, 3D culture systems can provide biological insights for translational clinical research in the fields of muscle physiology and metabolism. These systems can enhance the cell culture environment by improving spatially-oriented cellular assemblies and morphological features closely mimicking the in vivo tissues/organs, since they promote strong interactions between cells and the extracellular matrix (ECM). However, the time-consuming and complex nature of 3D traditional culture techniques pose a challenge to the widespread adoption of 3D systems. Herein, a bench protocol is presented for creating an innovative, promptly assembled and user-friendly culture platform for the magnetic 3D bioprinting of skeletal muscle spheroids. Our protocol findings revealed consistent morphological outcomes and the functional development of skeletal muscle tissue, as evidenced by the expression of muscle-specific contractile proteins and myotubes and the responsiveness to stimulation with cholinergic neurotransmitters. This proof-of-concept protocol confirmed the future potential for further validation and application of spheroid-based assays in human skeletal muscle research.
在过去十年中，基于磁性三维（3D）的细胞培养系统的应用得到了迅速发展。就骨骼肌而言，3D培养系统可为肌肉生理学和代谢领域的转化临床研究提供生物学见解。这些系统可以通过改善空间定向的细胞组装和紧密模拟体内组织/器官的形态特征来增强细胞培养环境，因为它们促进了细胞与细胞外基质（ECM）之间的强相互作用。然而，3D传统培养技术耗时且复杂的性质对3D系统的广泛采用构成了挑战。在此，我们提出了一个实验方案，用于创建一个创新的、快速组装且用户友好的培养平台，用于骨骼肌球体的磁性3D生物打印。我们的实验方案结果显示出一致的形态学结果以及骨骼肌组织的功能发育，肌肉特异性收缩蛋白和肌管的表达以及对胆碱能神经递质刺激的反应性证明了这一点。这个概念验证方案证实了基于球体的检测方法在人类骨骼肌研究中进一步验证和应用的未来潜力。
