Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador 40296-710, Bahia, Brazil; Department of Propaedeutics, School of Dentistry of the Federal University of Bahia, Salvador 40110-150, Bahia, Brazil.
Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador 40296-710, Bahia, Brazil; Department of Pathology, School of Medicine of the Federal University of Bahia, Salvador 40110-909, Bahia, Brazil.
SLAS Discov. 2024 Jun;29(4):100158. doi: 10.1016/j.slasd.2024.100158. Epub 2024 May 1.
3D in vitro systems offer advantages over the shortcomings of two-dimensional models by simulating the morphological and functional features of in vivo-like environments, such as cell-cell and cell-extracellular matrix interactions, as well as the co-culture of different cell types. Nevertheless, these systems present technical challenges that limit their potential in cancer research requiring cell line- and culture-dependent standardization. This protocol details the use of a magnetic 3D bioprinting method and other associated techniques (cytotoxicity assay and histological analysis) using oral squamous cell carcinoma cell line, HSC3, which offer advantages compared to existing widely used approaches. This protocol is particularly timely, as it validates magnetic bioprinting as a method for the rapid deployment of 3D cultures as a tool for compound screening and development of heterotypic cultures such as co-culture of oral squamous cell carcinoma cells with cancer-associated fibroblasts (HSC3/CAFs).
3D 体外系统通过模拟类似于体内的环境的形态和功能特征，例如细胞-细胞和细胞-细胞外基质相互作用以及不同细胞类型的共培养，提供了优于二维模型的优势。然而，这些系统存在技术挑战，限制了它们在需要依赖细胞系和培养物进行标准化的癌症研究中的潜力。本协议详细介绍了使用磁性 3D 生物打印方法和其他相关技术（细胞毒性测定和组织学分析）的方法，该方法使用口腔鳞状细胞癌细胞系 HSC3，与现有广泛使用的方法相比具有优势。本协议特别及时，因为它验证了磁性生物打印作为一种快速部署 3D 培养物的方法，可作为化合物筛选和异质培养（例如口腔鳞状细胞癌细胞与癌症相关成纤维细胞的共培养）的工具。
