• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-218-5p 通过 LRIG1 促进乳腺癌的恶性行为。

MicroRNA-218-5p accelerates malignant behaviors of breast cancer through LRIG1.

机构信息

School of Medicine, Tongji University, China.

Department of Thyroid and Breast Surgery, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, China.

出版信息

Clinics (Sao Paulo). 2023 Oct 28;78:100302. doi: 10.1016/j.clinsp.2023.100302. eCollection 2024.

DOI:10.1016/j.clinsp.2023.100302
PMID:39491279
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10632409/
Abstract

OBJECTIVE

MicroRNAs play crucial roles in the pathogenesis of cancers. MiRNA-218-5p may act as either an oncogene or a tumor suppressor, but its role in the pathogenesis of Breast Cancer (BC) remains unclear.

METHODS

Infiltrative breast ductal carcinoma as well as corresponding adjacent normal samples were collected from 30 patients. Mimics and inhibitors of miRNA-218-5p or corresponding negative controls were transfected into BC cells. miRNA-218-5p expression was detected by quantitative PCR. The effects of miRNA-218-5p on the malignant behaviors of BC were assessed. Dual-luciferase reporter assay was employed to evaluate the binding of miRNA-218-5p to LRIG1.

RESULTS

BC tissues showed higher miRNA-218-5p expression as compared to the adjacent normal tissues. Ectopic miRNA-218-5p expression accelerated the cell cycle, cell growth and migration of BC, while repressed cell apoptosis. Interestingly, ectopic miRNA-218-5p expression down-regulated LRIG1 expression, and miRNA-218-5p could bind to LRIG1. Also, our study indicated that miRNA-218-5p up-regulated ErbB2 and EGFR expression by targeting LRIG1, suggesting that the LRIG1-mediated signaling pathway contributed to the pro-tumor effects of miRNA-218-5p on BC.

CONCLUSION

MiRNA-218-5p up-regulates ErbB2 and EGFR expression by suppressing LRIG1 expression, thus promoting the malignant behaviors of BC. miRNA-218-5p may exert a pro-tumor effect on BC and serve as a therapeutic target for BC treatment.

摘要

目的

microRNAs 在癌症的发病机制中发挥着关键作用。miRNA-218-5p 可能作为癌基因或肿瘤抑制因子发挥作用，但它在乳腺癌（BC）发病机制中的作用尚不清楚。

方法

收集 30 例浸润性乳腺导管癌及相应的相邻正常组织。将 miRNA-218-5p 的模拟物和抑制剂或相应的阴性对照转染到 BC 细胞中。采用定量 PCR 检测 miRNA-218-5p 的表达。评估 miRNA-218-5p 对 BC 恶性行为的影响。采用双荧光素酶报告基因检测评估 miRNA-218-5p 与 LRIG1 的结合。

结果

与相邻正常组织相比，BC 组织中 miRNA-218-5p 的表达水平更高。外源性 miRNA-218-5p 表达加速了 BC 细胞周期、细胞生长和迁移，同时抑制了细胞凋亡。有趣的是，外源性 miRNA-218-5p 表达下调了 LRIG1 的表达，miRNA-218-5p 可以与 LRIG1 结合。此外，我们的研究表明，miRNA-218-5p 通过靶向 LRIG1 下调 ErbB2 和 EGFR 的表达，提示 LRIG1 介导的信号通路有助于 miRNA-218-5p 对 BC 的促肿瘤作用。

结论

miRNA-218-5p 通过抑制 LRIG1 的表达而上调 ErbB2 和 EGFR 的表达，从而促进 BC 的恶性行为。miRNA-218-5p 可能对 BC 发挥促肿瘤作用，并可作为 BC 治疗的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/f2ff91364939/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/2055728d5539/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/9b9125d3ba6d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/6f36ff1a27e0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/e732a765a764/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/f220aa491b78/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/f2ff91364939/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/2055728d5539/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/9b9125d3ba6d/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/6f36ff1a27e0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/e732a765a764/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/f220aa491b78/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bad/10632409/f2ff91364939/gr6.jpg

相似文献

1
MicroRNA-218-5p accelerates malignant behaviors of breast cancer through LRIG1.miR-218-5p 通过 LRIG1 促进乳腺癌的恶性行为。
Clinics (Sao Paulo). 2023 Oct 28;78:100302. doi: 10.1016/j.clinsp.2023.100302. eCollection 2024.
2
The Tumor-Suppressive Role of miR-204-5p Through Targeting Ezrin in Breast Cancer: Experimental Evidence From Cell Lines and Clinical Samples.miR-204-5p通过靶向埃兹蛋白在乳腺癌中的肿瘤抑制作用：来自细胞系和临床样本的实验证据
Clin Breast Cancer. 2025 Jul;25(5):e588-e596. doi: 10.1016/j.clbc.2025.02.013. Epub 2025 Mar 19.
3
Caveolin-1 inhibits the proliferation and invasion of lung adenocarcinoma via EGFR degradation.小窝蛋白-1通过表皮生长因子受体（EGFR）降解抑制肺腺癌的增殖和侵袭。
Sci Rep. 2025 Jul 1;15(1):21654. doi: 10.1038/s41598-025-05259-8.
4
MicroRNA-338-5p plays a tumor suppressor role in glioma through inhibition of the MAPK-signaling pathway by binding to FOXD1.miR-338-5p 通过与 FOXD1 结合抑制 MAPK 信号通路，在胶质瘤中发挥肿瘤抑制作用。
J Cancer Res Clin Oncol. 2018 Dec;144(12):2351-2366. doi: 10.1007/s00432-018-2745-y. Epub 2018 Sep 17.
5
Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer.SAE1上调驱动肿瘤发生并与乳腺癌不良临床预后相关。
Breast J. 2024 Jun 30;2024:2981722. doi: 10.1155/2024/2981722. eCollection 2024.
6
LINC01094 targets miR-1266-5p to halt neoplasm progression of cervical cancer.LINC01094靶向miR-1266-5p以阻止宫颈癌的肿瘤进展。
J Obstet Gynaecol. 2025 Dec;45(1):2522866. doi: 10.1080/01443615.2025.2522866. Epub 2025 Jul 16.
7
Post-transcriptional regulation of tumor suppressor gene lncRNA CARMN via mA modification and miRNA regulation in cervical cancer.抑癌基因 lncRNA CARMN 通过 mA 修饰和 miRNA 调控在宫颈癌中的转录后调控。
J Cancer Res Clin Oncol. 2023 Sep;149(12):10307-10318. doi: 10.1007/s00432-023-04893-x. Epub 2023 Jun 5.
8
CircSEPT9 promotes breast cancer progression by regulating PTBP3 expression via sponging miR-625-5p.环状 RNA SEPT9 通过海绵吸附 miR-625-5p 调控 PTBP3 表达促进乳腺癌进展。
Thorac Cancer. 2024 Apr;15(10):808-819. doi: 10.1111/1759-7714.15252. Epub 2024 Feb 26.
9
circ-NOLC1 inhibits the development of cervical cancer by regulating miR-330-5p-PALM signaling axis.环状非编码RNA NOLC1通过调控miR-330-5p-PALM信号轴抑制宫颈癌的发展。
Hereditas. 2025 Jun 18;162(1):108. doi: 10.1186/s41065-025-00478-5.
10
Circular RNA circHSPA8 Aggravates Metastasis by Acting as a Competitive Inhibitor of miR-195-5p to Upregulate WNT3A Expression in Breast Cancer.环状RNA circHSPA8通过作为miR-195-5p的竞争性抑制剂上调WNT3A表达促进乳腺癌转移。
J Cell Mol Med. 2025 Mar;29(6):e70499. doi: 10.1111/jcmm.70499.

引用本文的文献

1
Differences in Starvation-Induced Autophagy Response and miRNA Expression Between Rat Mammary Epithelial and Cancer Cells: Uncovering the Role of miR-218-5p.大鼠乳腺上皮细胞与癌细胞在饥饿诱导的自噬反应和miRNA表达上的差异：揭示miR-218-5p的作用
Cancers (Basel). 2025 Jul 23;17(15):2446. doi: 10.3390/cancers17152446.

本文引用的文献

1
Identification of markers for predicting prognosis and endocrine metabolism in nasopharyngeal carcinoma by miRNA-mRNA network mining and machine learning.通过 miRNA-mRNA 网络挖掘和机器学习鉴定预测鼻咽癌预后和内分泌代谢的标志物。
Front Endocrinol (Lausanne). 2023 Jul 19;14:1174911. doi: 10.3389/fendo.2023.1174911. eCollection 2023.
2
Identification of a glycolysis-related miRNA Signature for Predicting Breast cancer Survival.鉴定与糖酵解相关的 miRNA 特征，用于预测乳腺癌患者的生存情况。
Mol Biotechnol. 2024 Aug;66(8):1988-2006. doi: 10.1007/s12033-023-00837-5. Epub 2023 Aug 3.
3
A novel miRNA-based signature as predictive tool of survival outcome of colorectal cancer patients.
一种基于新型微小RNA的特征作为结直肠癌患者生存结局的预测工具。
Chem Biol Drug Des. 2023 Nov;102(5):1024-1033. doi: 10.1111/cbdd.14301. Epub 2023 Aug 2.
4
MicroRNA-30c-5p arrests bladder cancer G2/M phase and suppresses its progression by targeting PRC1-mediated blocking of CDK1/Cyclin B1 axis.微小 RNA-30c-5p 通过靶向 PRC1 介导的 CDK1/细胞周期蛋白 B1 轴阻断来阻滞膀胱癌 G2/M 期并抑制其进展。
Cell Signal. 2023 Oct;110:110836. doi: 10.1016/j.cellsig.2023.110836. Epub 2023 Aug 1.
5
The mirrored cationic peptide as miRNA vehicle for efficient lung cancer therapy.作为用于高效肺癌治疗的微小RNA载体的镜像阳离子肽。
MedComm (2020). 2023 Jul 28;4(4):e273. doi: 10.1002/mco2.273. eCollection 2023 Aug.
6
MicroRNA-Based Discovery of Biomarkers, Therapeutic Targets, and Repositioning Drugs for Breast Cancer.基于 microRNA 的乳腺癌生物标志物、治疗靶点和再定位药物的发现。
Cells. 2023 Jul 23;12(14):1917. doi: 10.3390/cells12141917.
7
Integrative analysis of mRNA and miRNA expression profiles and somatic variants in oxysterol signaling in early-stage luminal breast cancer.早期腔面型乳腺癌甾醇信号中 mRNA 和 miRNA 表达谱及体细胞变异的综合分析。
Mol Oncol. 2023 Oct;17(10):2074-2089. doi: 10.1002/1878-0261.13495. Epub 2023 Aug 18.
8
Targeting miRNA and using miRNA as potential therapeutic options to bypass resistance in pancreatic ductal adenocarcinoma.针对 miRNA 并将其用作潜在的治疗选择，以绕过胰腺导管腺癌的耐药性。
Cancer Metastasis Rev. 2023 Sep;42(3):725-740. doi: 10.1007/s10555-023-10127-w. Epub 2023 Jul 25.
9
microRNA-944 inhibits breast cancer cell proliferation and promotes cell apoptosis by reducing SPP1 through inactivating the PI3K/Akt pathway.microRNA-944 通过抑制 PI3K/Akt 通路减少 SPP1 从而抑制乳腺癌细胞增殖并促进细胞凋亡。
Apoptosis. 2023 Dec;28(11-12):1546-1563. doi: 10.1007/s10495-023-01870-0. Epub 2023 Jul 24.
10
GABPB1-AS1 acts as a tumor suppressor and inhibits non-small cell lung cancer progression by targeting miRNA-566/F-box protein 47.GABPB1-AS1 通过靶向 miRNA-566/F-box 蛋白 47 发挥肿瘤抑制因子的作用，抑制非小细胞肺癌的进展。
Oncol Res. 2022 Nov 10;29(6):401-409. doi: 10.32604/or.2022.025262. eCollection 2021.