Kreplin Laura Zoe, Arumugam Senthil
Monash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Melbourne, VIC 3800, Australia; European Molecular Biology Laboratory Australia (EMBL Australia), Monash University, Clayton, Melbourne, VIC 3800, Australia.
Monash Biomedicine Discovery Institute, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Melbourne, VIC 3800, Australia; European Molecular Biology Laboratory Australia (EMBL Australia), Monash University, Clayton, Melbourne, VIC 3800, Australia.
Curr Opin Cell Biol. 2023 Oct 27;85:102272. doi: 10.1016/j.ceb.2023.102272.
Research in the areas of organelle dynamics, cytoskeletal interactions, membrane protrusions, and cell motility relies heavily on live-cell imaging. These structures continuously move about in complex patterns and imaging them live at sufficient temporal resolutions as well as for durations long enough to extract significant number of events is an absolute necessity. Capturing most of the sub-cellular dynamics in whole cell volumes was beyond reach due to the lack of balance between reduced photo-toxicity, time resolution, and the required spatial resolution in dominant imaging modalities like point scanning confocal and spinning disc confocal microscopy. In the last few years, a plethora of light-sheet geometries have emerged, pushing the limits of measurements. In this review, we will focus on a subset of light-sheet modalities that are most suited to studying live, sub-cellular dynamics in whole-cell volumes.
在细胞器动力学、细胞骨架相互作用、膜突出和细胞运动等领域的研究严重依赖活细胞成像。这些结构以复杂的模式持续移动，要以足够的时间分辨率对其进行实时成像，并持续足够长的时间以提取大量事件，这是绝对必要的。由于在诸如点扫描共聚焦显微镜和转盘共聚焦显微镜等主流成像方式中，降低光毒性、时间分辨率和所需空间分辨率之间缺乏平衡，因此在全细胞体积中捕捉大多数亚细胞动力学是无法实现的。在过去几年中，出现了大量的光片几何形状，突破了测量的极限。在这篇综述中，我们将重点关注最适合研究全细胞体积中活细胞亚细胞动力学的一部分光片模式。
