Zhao Wanli, Wu Junzhi, Tian Mei, Xu Shu, Hu Shuaiya, Wei Zhiyan, Lin Guyin, Tang Liang, Wang Ruiyang, Feng Boya, Wang Bi, Lyu Hui, Paetz Christian, Feng Xu, Xue Jia-Yu, Li Pirui, Chen Yu
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Jiangsu Province Engineering Research Center of Eco-cultivation and High-value Utilization of Chinese Medicinal Materials, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), 210014 Nanjing, China.
Nanjing University of Chinese Medicine, 210023 Nanjing, China.
Hortic Res. 2024 Mar 8;11(4):uhae042. doi: 10.1093/hr/uhae042. eCollection 2024 Apr.
Phenylphenalenones (PhPNs), phytoalexins in wild bananas (Musaceae), are known to act against various pathogens. However, the abundance of PhPNs in many Musaceae plants of economic importance is low. Knowledge of the biosynthesis of PhPNs and the application of biosynthetic approaches to improve their yield is vital for fighting banana diseases. However, the processes of PhPN biosynthesis, especially those involved in methylation modification, remain unclear. is a herbaceous plant belonging to Musaceae, and due to the abundant PhPNs, their biosynthesis in has been the subject of much attention. In this study, we assembled a telomere-to-telomere gapless genome of as the reference, and further integrated transcriptomic and metabolomic data to mine the candidate genes involved in PhPN biosynthesis. To elucidate the diversity of PhPNs in , three screened -methyltransferases (Ml01G0494, Ml04G2958, and Ml08G0855) by phylogenetic and expressional clues were subjected to enzymatic assays. The results show that the three were all novel -methyltransferases involved in the biosynthesis of PhPN phytoalexins, among which Ml08G0855 was proved to function as a multifunctional enzyme targeting multiple hydroxyl groups in PhPN structure. Moreover, we tested the antifungal activity of PhPNs against and found that the methylated modification of PhPNs enhanced their antifungal activity. These findings provide valuable genetic resources in banana breeding and lay a foundation for improving disease resistance through molecular breeding.
苯并菲酮（PhPNs）是野生香蕉（芭蕉科）中的植物抗毒素，已知其对多种病原体具有作用。然而，许多具有经济重要性的芭蕉科植物中PhPNs的含量较低。了解PhPNs的生物合成以及应用生物合成方法提高其产量对于防治香蕉病害至关重要。然而，PhPNs的生物合成过程，尤其是那些涉及甲基化修饰的过程，仍不清楚。[此处可能缺失植物名称]是一种属于芭蕉科的草本植物，由于其PhPNs含量丰富，其生物合成一直备受关注。在本研究中，我们组装了[此处可能缺失植物名称]的端粒到端粒无间隙基因组作为参考，并进一步整合转录组和代谢组数据以挖掘参与PhPN生物合成的候选基因。为了阐明[此处可能缺失植物名称]中PhPNs的多样性，通过系统发育和表达线索筛选出的三种甲基转移酶（Ml01G0494、Ml04G2958和Ml08G0855）进行了酶活性测定。结果表明，这三种都是参与PhPN植物抗毒素生物合成的新型甲基转移酶，其中Ml08G0855被证明是一种针对PhPN结构中多个羟基的多功能酶。此外，我们测试了PhPNs对[此处可能缺失病原体名称]的抗真菌活性，发现PhPNs的甲基化修饰增强了它们的抗真菌活性。这些发现为香蕉育种提供了有价值的遗传资源，并为通过分子育种提高抗病性奠定了基础。
