Kim Bo Kyeong, Kim Ki-Uk, Kim Jisu, Jang Hyunjun, Min Hyeyoung
College of Pharmacy, Chung-Ang University, 84 Heukseokro, Dongjakgu, Seoul, 06974 Korea.
Food Sci Biotechnol. 2024 Jun 28;33(15):3607-3616. doi: 10.1007/s10068-024-01617-w. eCollection 2024 Dec.
Ginsenosides, constituting 2-3% of Meyer, are noteworthy for their anticancer and antioxidant effects. Despite demonstrating promise in various diseases, their specific impact on age-related macular degeneration (AMD) remains unclear. This research investigates whether ginsenosides can inhibit the progression of dry AMD and explores the mechanisms by which they influence apoptosis, providing insight into their regulatory role in programmed cell death. Human retinal pigment epithelial (ARPE-19) cells were pre-treated with ginsenosides, followed by induction of oxidative stress using hydrogen peroxide. Pre-treatment with 20(S)-ginsenoside Rg3 significantly increased cell viability and reduced apoptotic markers, including Annexin V, Bax, Bim S, cleaved caspase 3, cleaved caspase 9, and cleaved PARP. Furthermore, 20(S)-ginsenoside Rg3 effectively diminished the activation of the ERK and NF-κB signaling pathways. 20(S)-ginsenoside Rg3 could be a good prevention for AMD by modulating apoptosis, offering valuable therapeutic insights for AMD.
人参皂苷占人参的2%-3%，因其抗癌和抗氧化作用而备受关注。尽管人参皂苷在各种疾病中显示出前景，但其对年龄相关性黄斑变性（AMD）的具体影响仍不清楚。本研究调查人参皂苷是否能抑制干性AMD的进展，并探索其影响细胞凋亡的机制，以深入了解其在程序性细胞死亡中的调节作用。用人参皂苷预处理人视网膜色素上皮（ARPE-19）细胞，然后用过氧化氢诱导氧化应激。用20(S)-人参皂苷Rg3预处理可显著提高细胞活力，并减少凋亡标志物，包括膜联蛋白V、Bax、Bim S、裂解的半胱天冬酶3、裂解的半胱天冬酶9和裂解的聚（ADP-核糖）聚合酶。此外，20(S)-人参皂苷Rg3有效降低了ERK和NF-κB信号通路的激活。20(S)-人参皂苷Rg3可能通过调节细胞凋亡对AMD起到良好的预防作用，为AMD提供有价值的治疗见解。
