Kalmobe Justin, Vildina Jacqueline Dikti, Boursou Djafsia, Menga Honore Ndouwe Tissebe, Kouam Simeon Fogue, Ndjonka Dieudonne
Department of Biological Sciences, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
Department of Parasitology and Parasitic Pathology, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
J Parasit Dis. 2024 Dec;48(4):891-903. doi: 10.1007/s12639-024-01701-2. Epub 2024 Aug 12.
Human onchocerciasis is caused by the filarial worm. is a parasite that forms nodules under the skin. The aim of this work was to assess the nematicidal activities of on and and to determine the phytochemical compounds. Nodules were collected from the umbilical region of infected cattle, dissected and male worms were cultured in RPMI-1640. Worms were incubated with different concentrations of extracts in RPMI-1640 and M9-buffer. Polyphenol, tannin and flavonoid contents of extract were determined by using gallic acid and rutin as standards. The anthelmintic effect of extract against was concentration dependent with LC of 20.71 µg/mL and 11.75 µg/mL after 48 and 72 h respectively. extract exerted concentration dependent lethal effects (LC = 2747 and LC = 31,937 µg/mL) against (Wild Type). Methanolic-methylene chloride (MeOH-CHCl) of extract exhibited high DPPH activity with an IC value of 15 µg/mL and 9 µg/mL for ascorbic acid. The highest activity in adult worms was observed with the MeOH (100: 0) and AcOEtMeOH fractions with LC values of 12.82 and 15.50 µg/mL respectively. EcOEtMeOH (8:2 v/v) was more effective (LC = 250 µg/mL) on WT of contains polyphenols (1015.05 and AcOEtMeOH = 893.60), flavonoids (25.35 and MeOH = 225.76) and tannins (401.37 and Hex = 788.89). showed in vitro nematicidal activity against and . could be used as an alternative anthelmintic for onchocerciasis treatment.
人类盘尾丝虫病由丝虫引起。它是一种在皮肤下形成结节的寄生虫。这项工作的目的是评估[提取物名称]对[寄生虫名称1]和[寄生虫名称2]的杀线虫活性，并确定其植物化学成分。从受感染牛的脐部收集结节，进行解剖，将雄虫培养于RPMI - 1640培养基中。将虫体与不同浓度的[提取物名称]提取物在RPMI - 1640培养基和M9缓冲液中孵育。以没食子酸和芦丁为标准测定提取物中的多酚、单宁和黄酮含量。[提取物名称]提取物对[寄生虫名称1]的驱虫作用呈浓度依赖性，48小时和72小时后的LC50分别为20.71μg/mL和11.75μg/mL。[提取物名称]提取物对[寄生虫名称2]（野生型）产生浓度依赖性致死作用（LC50分别为2747和31937μg/mL）。[提取物名称]的甲醇 - 二氯甲烷（MeOH - CHCl）提取物表现出较高的DPPH活性，抗坏血酸的IC50值为15μg/mL和9μg/mL。在成虫中，MeOH（100:0）和乙酸乙酯 - 甲醇（AcOEtMeOH）馏分的活性最高，LC50值分别为12.82和15.50μg/mL。乙酸乙酯 - 甲醇（8:2 v/v）对[寄生虫名称2]野生型更有效（LC50 = 250μg/mL），[提取物名称]含有多酚（1015.05，乙酸乙酯 - 甲醇 = 893.60）、黄酮（25.35，甲醇 = 225.76）和单宁（401.37，己烷 = 788.89）。[提取物名称]对[寄生虫名称1]和[寄生虫名称2]表现出体外杀线虫活性。[提取物名称]可作为治疗盘尾丝虫病的替代驱虫药。
