Anti-Onchocerca and Anti-Caenorhabditis Activity of a Hydro-Alcoholic Extract from the Fruits of Acacia nilotica and Some Proanthocyanidin Derivatives.

fruits with high tannin content are used in the northern parts of Cameroon as anti-filarial remedies by traditional healers. In this study, the hydro-alcoholic fruit extract (crude extract (CE)) and, one of the main constituents in its most active fractions, (+)-catechin-3--gallate (CG), as well as four related proanthocyanidins, (-)-epicatechin-3--gallate (ECG), (+)-gallocatechin (GC), (-)-epigallocatechin (EGC) and (-)-epigallocatechin-3--gallate (EGCG), were assessed for their potential in vitro anthelmintic properties against the free-living model organism and against the cattle filarial parasite . Worms were incubated in the presence of different concentrations of fruit extract, fractions and pure compounds. The effects on mortality were monitored after 48 h. The plant extract and all of the pure tested compounds were active against (LC ranging from 1.2 to 11.5 µg/mL on males) and (LC ranging from 33.8 to 350 µg/mL on wild type). While high LC were required for the effects of the compounds on , very low LC were required against . Importantly, tests for acute oral toxicity (lowest dose: 10 mg/kg) in Wistar rats demonstrated that crude extract and pure compounds were non-toxic and safe to use. Additionally, the results of cytotoxicity tests with the Caco-2 cell line (CC ranging from 47.1 to 93.2 µg/mL) confirmed the absence of significant toxicity of the crude extract and pure compounds. These results are in good accordance with the use of against nematode infections by traditional healers, herdsmen and pastoralists in Cameroon.