Golpour Monireh, Sohbatzadeh Farshad, Alimohammadi Mina, Yazdani Zahra, Fattahi Sadegh, Zaboli Ehsan, Rafiei Alireza, Bekeschus Sander
Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Molecular and Cell Biology Research Center (MCBRC), Hemoglobinopathy Institute, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Int J Mol Cell Med. 2024;13(3):248-258. doi: 10.22088/IJMCM.BUMS.13.3.248.
Chronic lymphocytic leukemia (CLL) is the most prevalent hematological cancer, with various medical interventions. In the recent decade, cold physical plasma has become an interesting agent for future cancer therapy. The goal of this study was to see whether cold physical plasma or cold physical plasma-treated liquid (PTL) affected integrin beta 3 (ITGB3) expression, which is hypothesized to mediate an interaction between cancer stem cells and the bone marrow microenvironment, in CLL patients' blood cells. The metabolic activity, cell death pattern, lipid oxidation and ITGB3 gene expression of these treatments was evaluated. Both direct cold physical plasma and PTL exposure enhanced lipid peroxidation in cells of CLL patients, but to a lesser extent in healthy participants. Furthermore, following 48h of cold physical plasma or PTL exposure, the metabolic activity of leukocytes was preferentially reduced in CLL patient leukocytes. In addition, cold physical plasma and PTL treatment elevated ITGB3 mRNA expression in CLL patients' leukocytes compared to untreated and healthy controls. Collectively, our study suggests selective effects of direct cold physical plasma and PTL exposure on blood leukocytes from leukemia patients, but further and more detailed studies are needed to provide additional rationales for such treatment options as future therapy.
慢性淋巴细胞白血病（CLL）是最常见的血液系统癌症，有多种医学干预措施。在最近十年中，冷物理等离子体已成为未来癌症治疗中一种引人关注的介质。本研究的目的是观察冷物理等离子体或冷物理等离子体处理液（PTL）是否会影响整合素β3（ITGB3）的表达，据推测该表达介导了癌症干细胞与骨髓微环境之间的相互作用，在CLL患者的血细胞中。对这些处理的代谢活性、细胞死亡模式、脂质氧化和ITGB3基因表达进行了评估。直接冷物理等离子体和PTL暴露均增强了CLL患者细胞中的脂质过氧化，但在健康参与者中程度较轻。此外，在冷物理等离子体或PTL暴露48小时后，CLL患者白细胞中的白细胞代谢活性优先降低。此外，与未处理的和健康对照相比，冷物理等离子体和PTL处理提高了CLL患者白细胞中ITGB3 mRNA的表达。总体而言，我们的研究表明直接冷物理等离子体和PTL暴露对白血病患者血液白细胞有选择性作用，但需要进一步更详细的研究，为将此类治疗方案作为未来治疗提供更多理论依据。
