Carrillo Heredero Alicia Maria, Segato Giulia, Menotta Simonetta, Butovskaya Elena, Borra Annalisa, Bertini Simone
Department of Veterinary Sciences, University of Parma, Strada del Taglio 10, Parma 43126, Italy.
Food and Feed Chemistry Department, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna "Bruno Ubertini" (IZSLER), via A. Bianchi 9, Brescia 25124, Italy.
ACS Omega. 2024 Oct 18;9(43):43649-43657. doi: 10.1021/acsomega.4c05899. eCollection 2024 Oct 29.
Ivermectin is a widely used broad-spectrum antiparasitic agent employed in both companion animals and livestock. While generally safe for mammals, its acute and chronic toxicity has been proven in many nontarget fish and insect species at low concentrations. The present study aimed to develop, validate, and test a more accurate method for ivermectin detection in various matrices (feces, soil, and sewage) for environmental studies. The method was validated using the Eurachem network. The applicability of the method was established at 1.5-500 μg/L for feces and 1.5-15 μg/L for sewage and soil. No dilution effect was observed at 5000 μg/L. The limit of detection was 0.66, 0.54, and 0.36 μg/kg for feces, soil, and sewage, respectively. The limit of quantification was set at 1.5 μg/kg and verified experimentally. Overall recovery was 92.27 ± 12.01% for feces and 96.19 ± 13.78% in soil, reaching a linearity of at least 0.99. The present method was successfully applied to 228 feces, 20 sewage, and 105 soil samples. This validated method enables the precise detection and quantification of ivermectin in the tested matrices, thereby envisaging potential applications in environmental monitoring studies with improved sensitivity and specificity.
伊维菌素是一种广泛应用于伴侣动物和家畜的广谱抗寄生虫药。虽然它对哺乳动物一般是安全的，但已证实其在低浓度下对许多非目标鱼类和昆虫物种具有急性和慢性毒性。本研究旨在开发、验证并测试一种更准确的方法，用于环境研究中检测各种基质（粪便、土壤和污水）中的伊维菌素。该方法使用欧洲化学网络进行了验证。该方法在粪便中的适用范围为1.5 - 500μg/L，在污水和土壤中的适用范围为1.5 - 15μg/L。在5000μg/L时未观察到稀释效应。粪便、土壤和污水的检测限分别为0.66、0.54和0.36μg/kg。定量限设定为1.5μg/kg并通过实验进行了验证。粪便的总体回收率为92.27±12.01%，土壤中的回收率为96.19±13.78%，线性至少达到0.99。本方法成功应用于228份粪便、20份污水和105份土壤样品。这种经过验证的方法能够精确检测和定量测试基质中的伊维菌素，从而设想在环境监测研究中具有提高的灵敏度和特异性的潜在应用。
