Zhang Zhen, Islam Mohammad Shafiqul, Xia Jiuzhi, Feng Xiangyang, Noman Muhammad, Wang Jing, Hao Zhongna, Qiu Haiping, Chai Rongyao, Cai Yingying, Wang Yanli, Wang Jiaoyu
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Molecular Biology of Crop Pathogens and Insects of MARA, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Front Plant Sci. 2024 Oct 15;15:1482934. doi: 10.3389/fpls.2024.1482934. eCollection 2024.
The rice blast disease, caused by the fungus , is a significant agricultural problem that adversely impacts rice production and food security. Understanding the precise molecular pathways involved in the interaction between the pathogen and its host is crucial for developing effective disease management strategies. This study examines the crucial function of the nucleolin MoNsr1 in regulating physiological functions. Δ deletion mutants showed reduced fungal growth, asexual sporulation, and pathogenicity compared to the wild-type. Mutants exhibited impaired conidial germination and appressoria formation, reducing infection progression. Additionally, Δ deletion mutant had less turgor pressure, confirming that MoNsr1 is essential for cell wall biogenesis and resistant to external stresses. Furthermore, Δ deletion mutant showed enhanced sensitivity to oxidative stress, reactive oxygen species, and cold tolerance. Our results offer a thorough understanding of the function of MoNsr1 in the virulence and stress-resilient capability in . These findings provide insights into the novel targets and contribute to the emergence of innovative approaches for managing rice blast disease.
由该真菌引起的稻瘟病是一个严重的农业问题，对水稻生产和粮食安全产生不利影响。了解病原体与其宿主之间相互作用所涉及的精确分子途径对于制定有效的病害管理策略至关重要。本研究考察了核仁蛋白MoNsr1在调节生理功能中的关键作用。与野生型相比，Δ缺失突变体的真菌生长、无性孢子形成和致病性均降低。突变体的分生孢子萌发和附着胞形成受损，从而降低了感染进程。此外，Δ缺失突变体的膨压较小，证实MoNsr1对于细胞壁生物合成和抵抗外部压力至关重要。此外，Δ缺失突变体对氧化应激、活性氧和耐寒性表现出增强的敏感性。我们的结果全面了解了MoNsr1在稻瘟病菌毒力和抗逆能力中的功能。这些发现为新靶点提供了见解，并有助于出现管理稻瘟病的创新方法。
