School of Chemistry, University of Edinburgh, Joseph Black Building, King's Buildings, Edinburgh, EH9 3FJ, UK.
School of Biological Sciences, University of Edinburgh, Roger Land Building, King's Buildings, Edinburgh, EH9 3FF, UK.
Chem Commun (Camb). 2024 Nov 19;60(93):13746-13749. doi: 10.1039/d4cc05182c.
Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC), we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction. We demonstrate that ArtiSt functions under ambient conditions with low catalyst loading. Furthermore, activity can be increased >20 fold by altering the protein scaffold.
我们使用一种通过硫胺素启发的 N-杂环卡宾（NHC）共价功能化的蛋白质支架，创建了一种人工 Stetterase（ArtiSt），它可以催化立体选择性的分子内 Stetter 反应。我们证明了 ArtiSt 在低催化剂负载下的环境条件下起作用。此外，通过改变蛋白质支架，活性可以增加 20 倍以上。
