Department of Pain, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyan, Hubei, China.
Vet Med Sci. 2024 Nov;10(6):e70090. doi: 10.1002/vms3.70090.
In this study, we explored the eco-friendly synthesis of photoluminescent CCDs employing a direct one-step pyrolysis process, utilizing natural Cortex Phellodendri Chinensis as the precursor material and studied their analgesic effect in mice. The synthesized carbon dots underwent comprehensive characterization through a range of spectroscopic and microscopic techniques. These included UV-Vis, FTIR, fluorescence spectroscopy and HR-TEM, DLS instruments. HR-TEM results exhibited the presence of homogenous spherical-shaped C-dots of about 3.3 nm without aggregates. Furthermore, the prepared CCDs were studied for their in vivo analgesic effect in mice by performing tail-immersion, hot plate and acetic acid writhing tests. Also, an MTT assay was performed to assess the in vitro cytotoxicity of CCDs against L929 cells. In vitro cytotoxicity studies revealed that L929 cells exhibited higher cell viability when treated with prepared CCDs. The cellular uptake studies revealed the phase contrast images of MG-63 cells at wavelength 488 nm clearly depicted the aggregation of green, fluorescent CCDs within the cells while leaving nuclei unobscured. In addition, to the best of our understanding, the results presented in this paper showed that CCDs exhibited an important analgesic effect and enhanced anti-nociceptive activity, which may be due to stimulation of the opioidergic system. Consequently, CCDs appear to be a viable analgesic alternative for traditional analgesic candidates in pain management.
在这项研究中，我们探索了利用天然黄柏作为前体材料，通过直接一步热解法合成光致发光 CCD 的环保方法，并研究了它们在小鼠中的镇痛作用。通过一系列光谱和显微镜技术对合成的碳点进行了全面表征。这些技术包括 UV-Vis、FTIR、荧光光谱和高分辨率透射电子显微镜（HR-TEM）、动态光散射（DLS）仪器。HR-TEM 结果表明，存在约 3.3nm 的均匀球形 C 点，没有聚集体。此外，通过进行尾浸、热板和醋酸扭体试验研究了制备的 CCD 在小鼠体内的镇痛作用。还进行了 MTT 测定以评估 CCD 对 L929 细胞的体外细胞毒性。体外细胞毒性研究表明，用制备的 CCD 处理时，L929 细胞表现出更高的细胞活力。细胞摄取研究显示，在 488nm 波长下，MG-63 细胞的相差对比图像清楚地描绘了细胞内绿色荧光 CCD 的聚集，而细胞核则清晰可见。此外，据我们所知，本文提出的结果表明，CCD 表现出重要的镇痛作用和增强的抗伤害感受活性，这可能是由于阿片能系统的刺激。因此，CCD 似乎是疼痛管理中传统镇痛候选物的可行替代方案。
