Segueni Khaoula, Chouikh Atef, Eddine Laouini Salah, Bouafia Abderrhmane, Laid Tlili Mohammed, Laib Ibtissam, Boudebia Ouafa, Khelef Yahia, Abdullah Mahmood M S, Abdullah Johar Amin Ahmed, Bin Emran Talha
Laboratory of Biology, Environment and Health (LBEH), El Oued University, El Oued, Algeria.
Department of Cellular and Molecular Biology, Faculty of Natural Science and Life, El Oued University, El Oued, Algeria.
Chem Biodivers. 2025 Mar;22(3):e202402284. doi: 10.1002/cbdv.202402284. Epub 2024 Nov 26.
This study investigates the wound-healing potential of Euphorbia guyoniana latex (EGL) in male Wistar rats, along with its biochemical composition and biological activities. Phytochemical analysis identified moderate levels of phenolics, flavonoids, and tannins, with HPLC revealing five phenolic compounds. EGL demonstrated strong antioxidant activity in DPPH assays, surpassing ascorbic acid in protecting red blood cells. Its performance in the ß-carotene-linoleic acid assay was robust, though its FRAP assay results were weaker. EGL also exhibited significant anti-inflammatory activity, comparable to Acetylsalicylic acid, and showed antibacterial effects against Listeria innocua. In Vivo, EGL-infused ointments accelerated wound healing, reducing epithelialization periods to 12-16 days, with a higher wound contraction rate compared to controls. The study concludes that EGL, rich in bioactive compounds, holds potential as a promising natural agent for wound healing, owing to its potent antioxidant, anti-inflammatory, and antibacterial properties.
本研究调查了 Guyon 大戟乳胶（EGL）对雄性 Wistar 大鼠的伤口愈合潜力，以及其生化组成和生物活性。植物化学分析确定了酚类、黄酮类和单宁的适度含量，高效液相色谱法揭示了五种酚类化合物。EGL 在 DPPH 试验中表现出强大的抗氧化活性，在保护红细胞方面超过了抗坏血酸。其在 β-胡萝卜素-亚油酸试验中的表现强劲，尽管其铁还原抗氧化能力（FRAP）试验结果较弱。EGL 还表现出显著的抗炎活性，与乙酰水杨酸相当，并对无害李斯特菌显示出抗菌作用。在体内，注入 EGL 的软膏加速了伤口愈合，将上皮形成期缩短至 12 - 16 天，与对照组相比伤口收缩率更高。该研究得出结论，富含生物活性化合物的 EGL，由于其强大的抗氧化、抗炎和抗菌特性，有望成为一种有前景的天然伤口愈合剂。
