Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Department of Pharmacology and Medical Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Daru. 2021 Jun;29(1):133-145. doi: 10.1007/s40199-021-00392-x. Epub 2021 May 9.
Punica granatum L. (pomegranate) with astringent activities and Matricaria chamomilla L. (chamomile) with anti-inflammatory and antioxidant properties are natural remedies used for various skin disorders, including wound healing.
This study was conducted to evaluate the individual and combined wound healing activity of the methanol extracts of pomegranate and chamomile flowers.
After preparing the menthol fraction of pomegranate and chamomile flowers, the content of total phenols, total tannins, and total flavonoids of fractions was measured. For standardization of pomegranate and chamomile fractions, Gallic acid and apigenin-7-O-glucoside contents of them were determined using high-performance liquid chromatography (HPLC). Moreover, their antioxidant activities were examined using DPPH and FRAP tests. The antimicrobial assay was performed against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Three different concentrations of methanol fraction of each plant and one combination dose of fractions were investigated for their wound healing activities in an excision wound model on the rats' dorsum. Finally, histopathological studies were done at the end of the experiment.
Phytochemical examinations showed high amounts of phenolic compounds in pomegranate flowers, while chamomile flower fractions contained a high amount of total flavonoids. Both fractions, especially pomegranate, had potent antioxidant activity. The best results for wound closure were observed 7 days after wound induction. All treated groups exhibited superior wound contraction compared to their placebo at all measurement times. The combined form of pomegranate and chamomile had better wound healing properties compared to a single therapy, especially on time earlier to wound induction.
This study represented high antioxidant and wound healing activities for methanol fraction of pomegranate and chamomile flowers, which could be related to their high content of phytochemicals. In comparison with single herb treatment, the combined form of these two fractions in lower concentrations accelerated wound closure.
具有收敛作用的石榴（pomegranate）和具有抗炎和抗氧化特性的母菊（chamomile）是用于治疗各种皮肤疾病（包括伤口愈合）的天然药物。
本研究旨在评估石榴和母菊花甲醇提取物的单独和联合的伤口愈合活性。
制备石榴和母菊花的薄荷醇馏分后，测量馏分中总酚、总单宁和总类黄酮的含量。为了对石榴和母菊馏分进行标准化，使用高效液相色谱法（HPLC）测定它们的没食子酸和芹菜素-7-O-葡萄糖苷的含量。此外，还使用 DPPH 和 FRAP 试验评估它们的抗氧化活性。采用抑菌试验对抗金黄色葡萄球菌、表皮葡萄球菌和铜绿假单胞菌。在大鼠背部切除伤模型中，研究了每种植物的甲醇馏分的三个不同浓度和一个馏分组合剂量的伤口愈合活性。最后，在实验结束时进行了组织病理学研究。
植物化学检查表明，石榴花中含有大量的酚类化合物，而母菊花馏分中含有大量的总类黄酮。两种馏分，特别是石榴，均具有很强的抗氧化活性。在伤口诱导后 7 天，观察到最佳的伤口闭合效果。与安慰剂相比，所有治疗组在所有测量时间点的伤口收缩都更好。与单一治疗相比，石榴和母菊的组合形式具有更好的伤口愈合特性，尤其是在更早的伤口诱导时间。
本研究代表了石榴和母菊花甲醇馏分具有高抗氧化和伤口愈合活性，这可能与其高含量的植物化学物质有关。与单一草药治疗相比，这两种馏分以较低浓度的组合形式可加速伤口闭合。
