体外评估注射用骨髓抽吸浓缩物与注射用富含血小板纤维蛋白比较。
In Vitro Assessment of Injectable Bone Marrow Aspirate Concentrates Compared to Injectable Platelet-Rich Fibrin.
机构信息
Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20, Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan.
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Shimane University, 89-1, Enya-cho, Izumo, Shimane, 693-8501, Japan.
出版信息
Tissue Eng Regen Med. 2024 Dec;21(8):1233-1243. doi: 10.1007/s13770-024-00677-7. Epub 2024 Nov 4.
BACKGROUND
Injectable platelet-rich fibrin (iPRF), a liquid form of PRF that is prepared from peripheral blood without anticoagulants, promotes tissue wound healing and regeneration. The present study focused on iPRF-like bone marrow aspirate concentrate (iBMAC) prepared without anticoagulant, and the regenerative potential of iPRF and iBMAC was compared in vitro.
METHODS
iPRF and iBMAC were prepared from the same New Zealand white rabbits. The cytocompatibility and regenerative potential of each concentrate were evaluated using primary rabbit gingival fibroblasts and osteoblasts.
RESULTS
Both gingival fibroblasts and osteoblasts treated with each concentrate exhibited excellent cell viability. Interestingly, compared to cells treated with iPRF, cells treated with iBMAC demonstrated significantly greater migration potential. Furthermore, higher mRNA levels of transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), and collagen I (COL1) were observed in gingival fibroblasts treated with iBMAC than in those treated with iPRF. Compared with osteoblasts treated with iPRF, osteoblasts treated with iBMAC exhibited greater differentiation potential, as indicated by increased osteocalcin (OCN) expression and mineralization capability.
CONCLUSION
The results of the in vitro study suggest that, compared with iPRF, iBMAC may promote wound healing and bone regeneration more effectively. However, further preclinical and clinical studies are needed to confirm the regenerative potential of iBMAC in the body.
背景
注射用富血小板纤维蛋白（iPRF）是一种无需抗凝剂即可从外周血中制备的 PRF 液体形式，可促进组织创伤愈合和再生。本研究专注于无需抗凝剂制备的 iPRF 样骨髓抽吸浓缩物（iBMAC），并比较了 iPRF 和 iBMAC 的体外再生潜力。
方法
从同一批新西兰白兔中制备 iPRF 和 iBMAC。使用原代兔牙龈成纤维细胞和成骨细胞评估每种浓缩物的细胞相容性和再生潜力。
结果
用每种浓缩物处理的牙龈成纤维细胞和成骨细胞的细胞活力均表现出色。有趣的是，与用 iPRF 处理的细胞相比，用 iBMAC 处理的细胞显示出显著更大的迁移潜力。此外，用 iBMAC 处理的牙龈成纤维细胞中转化生长因子-β（TGF-β）、血管内皮生长因子（VEGF）和胶原蛋白 I（COL1）的 mRNA 水平明显高于用 iPRF 处理的细胞。与用 iPRF 处理的成骨细胞相比，用 iBMAC 处理的成骨细胞表现出更大的分化潜力，表现为骨钙素（OCN）表达和矿化能力增加。
结论
体外研究的结果表明，与 iPRF 相比，iBMAC 可能更有效地促进伤口愈合和骨再生。然而，需要进一步的临床前和临床研究来确认 iBMAC 在体内的再生潜力。
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