Laboratory of Farm Animal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Veterinary Service Station, Hinode Dairy Farming Cooperative, Ibaraki, Japan.
J Vet Med Sci. 2024 Dec 1;86(12):1294-1298. doi: 10.1292/jvms.24-0320. Epub 2024 Nov 4.
A 35-month-old Japanese Black cow was presented to a local veterinarian with a main complaint of premature labor. There was no swelling of lymph nodes on the body surface and no palpable mass on rectal examination. Although atypical lymphocytes were observed in the peripheral blood, no lymphocytosis was recorded. On Day 12, the cow developed hindlimb paresis and was euthanized. Necropsy revealed swelling of some lymph nodes, and white nodules in uterus and right atrial appendage. Histopathological examination revealed B-cell lymphoma. In clonality analysis of peripheral blood and tumor tissue, monoclonal proliferation was detected by PCR for immunoglobulin light chain (IgL) λ, suggesting neoplastic proliferation of B-cell. IgL-PCR is thought to be a possible tool for diagnosis of B-cell lymphoma.
一头 35 月龄的日本黑牛因早产被带到当地兽医处就诊。体表淋巴结无肿胀，直肠检查无触诊肿块。外周血虽观察到异型淋巴细胞，但未记录到淋巴细胞增多。第 12 天，牛出现后肢瘫痪，被安乐死。剖检发现部分淋巴结肿大，子宫和右心房附件有白色小结节。组织病理学检查显示 B 细胞淋巴瘤。在外周血和肿瘤组织的克隆性分析中，通过免疫球蛋白轻链（IgL）λ的 PCR 检测到单克隆增殖，提示 B 细胞的肿瘤性增殖。IgL-PCR 被认为是诊断 B 细胞淋巴瘤的一种可能工具。
