Man S F, Hulbert W C, Mok K, Ryan T, Thomson A B
J Appl Physiol (1985). 1986 Feb;60(2):416-26. doi: 10.1152/jappl.1986.60.2.416.
The bioelectric and barrier properties of the tracheal epithelium in nose-breathing dogs and in dogs that had been exposed for 75 min to compressed air or to two high concentrations of SO2 were measured and compared. We also studied tissues that had been treated with chloroform. Based on a model of restrictive diffusion we demonstrated heteropores (6 and 250 A) in the control tissues. Bioelectric changes due to 100-ppm SO2 were minimal. After exposure to 500 ppm SO2, adverse changes in the bioelectric properties were focal; they were marked in 8 out of 12 animals but were less striking in the other 4. Nonelectrolyte permeability increased with an increase in SO2 concentrations. Small pores were still present in the tissues severely affected by SO2 but they were absent in chloroform-treated tissues. Scanning electron microscopy of tissues from animals exposed to 500 ppm SO2 showed that in the same dog tissue appearance varied from normal to one of repair (normal bioelectric properties) or one of marked exfoliation of ciliated cells (abnormal bioelectric measurements).
对经鼻呼吸的犬以及暴露于压缩空气或两种高浓度二氧化硫中75分钟的犬的气管上皮的生物电和屏障特性进行了测量和比较。我们还研究了用氯仿处理过的组织。基于限制性扩散模型，我们在对照组织中证实了存在异质孔（6埃和250埃）。100 ppm二氧化硫引起的生物电变化极小。暴露于500 ppm二氧化硫后，生物电特性的不良变化是局部性的；12只动物中有8只表现明显，但另外4只则不太显著。非电解质通透性随二氧化硫浓度的增加而增加。在受二氧化硫严重影响的组织中仍存在小孔，但在氯仿处理过的组织中则不存在。对暴露于500 ppm二氧化硫的动物组织进行扫描电子显微镜检查显示，在同一只犬的组织中，外观从正常到修复状态（正常生物电特性）或明显的纤毛细胞脱落状态（异常生物电测量）各不相同。
