Artimani Tayebe, Asl Sara Soleimani, Amiri Iraj, Pilehvari Shamim, Yavangi Mahnaz, Mohammadpour Nooshin, Moravej Fahimeh Ghasemi
Fertility and Infertility Research Center, Hamadan University of Medical Sciences, Hamadan, 6517838736, Iran.
Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Reprod Sci. 2024 Dec;31(12):3870-3879. doi: 10.1007/s43032-024-01744-z. Epub 2024 Nov 4.
Platelet-rich plasma (PRP) has been suggested for the improvement of endometrial growth and receptivity in the patients with recurrent implantation failure (RIF). The aim of present study was to investigate the impact of different concentration of PRP on the expression of genes involved in the endometrial receptivity in the human endometrial cells from RIF and controls with thin and normal endometrium in vitro. In this cross-sectional study, endometrial biopsies were obtained from 14 healthy fertile women and 14 women with RIF. Endometrial cells from 4 different group (RIF and control with endometrial thickness < 7 mm and > 7 mm) were cultured with three different concentration of PRP 3%, 5% and 10%. Expression of leukemia inhibitory factor (LIF), COX2 and P53, estrogen receptors (ERs) and progesterone receptors (PRs) genes were measured using quantitative real-time polymerase chain reaction (PCR). Protein expression levels of LIF, COX2 and p53 were evaluated using Western Blot method (WB). There was a significant decrease in the expression of PROA/b, ER2/b, LIF/b, COX2/b and P53/b genes in the RIF groups compared to the controls. Treatment with 5% and 10% PRP caused a significant increase in the gene expression of PRs, ERs, LIF/b, COX2/b and p53 in the RIF groups. Moreover, protein expression of COX2/b, LIF/b and p53/b increased following treatment with PRP in the RIF group with the endometrium thickness < 7 mm. PRP enhances expression of LIF, COX2, p53, ERs and PRs in the RIF patients with thin endometrium which may improve endometrium receptivity.
富血小板血浆（PRP）已被提议用于改善反复种植失败（RIF）患者的子宫内膜生长和容受性。本研究的目的是在体外研究不同浓度的PRP对RIF患者以及子宫内膜薄和正常的对照者的人子宫内膜细胞中参与子宫内膜容受性的基因表达的影响。在这项横断面研究中，从14名健康的可育女性和14名RIF女性中获取子宫内膜活检样本。来自4个不同组（RIF以及子宫内膜厚度<7mm和>7mm的对照）的子宫内膜细胞用三种不同浓度的PRP（3%、5%和10%）进行培养。使用定量实时聚合酶链反应（PCR）测量白血病抑制因子（LIF）、COX2和P53、雌激素受体（ERs）和孕激素受体（PRs）基因的表达。使用蛋白质印迹法（WB）评估LIF、COX2和p53的蛋白质表达水平。与对照组相比，RIF组中PROA/b、ER2/b、LIF/b、COX2/b和P53/b基因的表达显著降低。用5%和10%的PRP处理导致RIF组中PRs、ERs、LIF/b、COX2/b和p53的基因表达显著增加。此外，在子宫内膜厚度<7mm的RIF组中，用PRP处理后COX2/b、LIF/b和p53/b的蛋白质表达增加。PRP可增强子宫内膜薄的RIF患者中LIF、COX2、p53、ERs和PRs的表达，这可能会改善子宫内膜容受性。
